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近年来,世界各国药品管理机构都一直非常重视生物技术药物引起免疫系统损伤的毒性病理学评价。骨髓检查在新药研发GLP毒理实验中一直处于很重要的地位,包括骨髓涂片和骨髓切片。骨髓涂片受外界主客观影响因素较大,所以判断一个新药是否能引起骨髓学的变化仍然以骨髓切片作为判断依据的重要指标。由于胸骨骨髓组织内含有大量的钙盐而显得非常坚硬,需要先对其脱钙后才可直接进行石蜡切片、HE染色。传统的硝酸脱钙液脱钙效果较好,但用该方法脱钙后在实际应用中HE染色质量欠佳,诸多文献报
In recent years, drug regulatory agencies in various countries all over the world have always attached great importance to the toxicological pathological evaluation of the immune system caused by biotech drugs. Bone marrow examination has been very important in the new drug development GLP toxicology experiments, including bone marrow smears and bone marrow slices. Bone marrow smear affected by the main subjective and objective factors, so to determine whether a new drug can cause bone marrow changes are still based on bone marrow slices as an important indicator of judgment. As the sternal bone marrow contains a lot of calcium and appears to be very hard, you need to decalcification before they can directly paraffin sections, HE staining. The decalcification effect of the traditional decalcification solution is better, but the decalcification method with this method has poor quality of HE staining in practical applications. Many papers