论文部分内容阅读
目的:探讨胃癌组织中烯醇化酶-α(enolase-α,ENO1)和肿瘤型丙酮酸激酶(tumor M2 pyruvate kinase,M2-PK)的表达、相互关系及其临床意义。方法:取兰州大学第一医院2009年6月至2010年10月石蜡包埋胃组织标本78例,其中胃癌55例,胃溃疡组织23例。免疫组织化学法检测胃癌组织和胃溃疡组织中ENO1、M2-PK的表达,分析两者的相互关系及临床意义。结果:胃癌组织中ENO1阳性表达率为67.3%(37/55),明显高于胃溃疡组的30.4%(7/23)(P<0.01);ENO1表达与胃癌分化程度、浸润深度、淋巴结转移及TNM分期均相关(均P<0.05);M2-PK在胃癌组织中的阳性表达率为78.2%(43/55),明显高于胃溃疡组织的39.1%(9/23)(P<0.01),M2-PK表达与胃癌分化程度、浸润深度相关(均P<0.05)。胃癌组织中ENO1与M2-PK的表达呈正相关(r=0.5729,P<0.05)。结论:胃癌组织ENO1和M2-PK的表达上调与胃癌的发生、发展可能有关,联合检测该两种蛋白在胃癌组织中的表达对判断预后有一定意义。
Objective: To investigate the expression, correlation and clinical significance of enolase-α (ENO1) and tumor M2 pyruvate kinase (M2-PK) in gastric cancer. Methods: From January 2009 to October 2010, 78 cases of paraffin-embedded gastric tissue specimens from the First Hospital of Lanzhou University were collected, including 55 cases of gastric cancer and 23 cases of gastric ulcer. The expression of ENO1 and M2-PK in gastric cancer and gastric ulcer tissue was detected by immunohistochemistry, and the correlation between them and clinical significance were analyzed. Results: The positive expression rate of ENO1 in gastric cancer was 67.3% (37/55), which was significantly higher than that in gastric ulcer group (30.4%, 7/23) (P <0.01). The expression of ENO1 was correlated with the differentiation, depth of invasion, lymph node metastasis (All P <0.05). The positive expression rate of M2-PK in gastric cancer was 78.2% (43/55), which was significantly higher than that in gastric ulcer (39.1%, 9/23) ). The expression of M2-PK was correlated with the degree of differentiation and the depth of invasion (all P <0.05). The expression of ENO1 and M2-PK in gastric cancer tissues was positively correlated (r = 0.5729, P <0.05). Conclusion: Upregulation of ENO1 and M2-PK in gastric cancer may be related to the occurrence and development of gastric cancer. The joint detection of the expression of these two proteins in gastric cancer has some significance in judging the prognosis.