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目的调查贵州地区静注海洛因成瘾者TT病毒感染状况。方法以公布的TTV第 1读码区序列设计两对寡核苷酸引物 ,用套式聚合酶链反应 (nPCR)检测 10 7例静注海洛因成瘾者血清中TTVDNA ,以 6 2例正常人为对照。同时用酶联免疫法则丙肝病毒抗体 (抗HCV) ,逆转录套式聚合酶链反应 (RT -nPCR)测庚肝病毒核糖核酸 (HGVRNA)。结果正常人TTVDNA阳性率 6 .45 %,海洛因成瘾者 2 5 .2 3 %。两组相比 ,差异有显著意义 (P <0 .0 0 5 )。成瘾者TTVDNA阳性率在男、女间无差异 ,与注毒时间长短无相关 (P >0 .5 )。 2 7例TTVDNA阳性成瘾者中 ,11例为单纯TTV感染 ,16例重叠HCV或HGV感染 ,其中TTV、HCV、HGV三重感染 8例 ,TTV重叠HCV5例 ,重叠HGV3例。结论贵州地区静注海洛因成瘾者中有TTV感染存在 ,感染率高低与性别、注毒时间长短无关。TTV可以单独感染 ,但与HCV、HGV的重叠感染率较高。
Objective To investigate the status of TT virus in intravenous heroin addicts in Guizhou. Methods Two pairs of oligonucleotide primers were designed according to the published sequence of the first reading region of TTV. TdnaNA in 107 sera heroin addicts was detected by nested polymerase chain reaction (nPCR) Control. At the same time, hepatitis C virus (HGVRNA) was detected by enzyme-linked immunosorbent assay (HCV) and reverse transcriptase-mediated polymerase chain reaction (RT -nPCR). Results The positive rate of TTVDNA was 6.45% in normal subjects and 25.33% in heroin addicts. There was significant difference between the two groups (P <0.05). The positive rate of TTVDNA in addicts was no difference between male and female, no correlation with the duration of injection (P> 0.05). Twenty-seven TTV DNA positive addicts were infected with TTV alone. Totally 16 patients were infected with HCV or HGV infection. Among them, 8 were TTV, HCV and HGV triple infection, 5 were HCV overlap TTV, and 3 were HGV overlap. Conclusion There is a presence of TTV infection among heroin addicts who were intravenously injected in Guizhou Province. The infection rate was not related to sex and duration of injection. TTV can be infected alone, but with HCV, HGV overlapping infection rate is higher.