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在家兔心脏冠状动脉结扎缺血/再灌流的损伤型模上,采用电子自旋共振(ESR)技术,直接测定整体家兔心肌缺血/再灌流早期自由基信号。自由基清除剂2-β-氨乙基异硫脲(AET)1.7 mg/kg,结扎前15 miniv,能使缺血30 min/再灌流2 min时,缺血心肌中自由基总浓度(10.6±4.1 nmol/g)明显低于对照组(18.0±2.1 nmol/g),而维拉帕米不能明显降低自由基生成量。AET还能减少再灌流期间脂质过氧化产物丙二醛生成量,降低血浆中CK,LDH的含量,改善ECG变化,从而使心肌梗塞的范围由对照组的31±4％降至12±2％。此外,AET可消除致死性室颤的发生。实验结果提示,自由基在缺血/再灌流损伤发生中起重要作用。AET对缩小心肌梗塞范围,消除室颤的发生具有良好的作用,因此可能是一类防治急性心肌梗塞及溶栓治疗或心脏手术后再灌流引起心律失常的有应用价值的新药。
Electron spin resonance (ESR) technique was used to determine the signal of free radicals in early stage of myocardial ischemia / reperfusion in rabbits. Radical scavenger 1.7 mg / kg 2-β-aminoethyl isothiourea (AET), 15 miniv before ligation, 30 min ischemia / reperfusion for 2 min, the total free radical concentration in ischemic myocardium ± 4.1 nmol / g) was significantly lower than the control group (18.0 ± 2.1 nmol / g), while verapamil did not significantly reduce the amount of free radicals. AET also reduced the malondialdehyde (MDA) production during lipid peroxidation during reperfusion, decreased the levels of CK and LDH in plasma, and improved ECG changes. The myocardial infarct size decreased from 31 ± 4% in the control group to 12 ± 2 %. In addition, AET can eliminate the occurrence of lethal ventricular fibrillation. The experimental results suggest that free radicals play an important role in the occurrence of ischemia / reperfusion injury. AET to narrow the scope of myocardial infarction, to eliminate the occurrence of ventricular fibrillation has a good effect, it may be a class of prevention and treatment of acute myocardial infarction and thrombolytic therapy or cardiopulmonary bypass after cardiac arrhythmia caused by new drugs of great value.