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舒洛芬(Suprofen)系新型镇痛药及前列腺素合成酶抑制剂。本文报道,用HPLC测定该原料药及其胶囊剂,方法简便、快速、稳定性好。方法:使用C_(18)键合反相硅胶柱,流动相为乙腈-pH3.0缓冲液(3:2)混合液,pH3.0缓冲液系用0.02M磷酸氢二钠液(Na_2HPO_4-7H_2O)再以0.01M枸橼酸调至pH2.97～3.04。流速为0.5ml/分,于UV254nm处检测。样品用甲醇溶解或提取,最后制成浓度为1mg/ml并含有内标4-硝基苯甲酸0.6mg/ml的甲醇液(标准品制备相同),每次注射2μl,随后测量峰高
Suprofen is a novel analgesic and prostaglandin synthase inhibitor. This article reports, by HPLC determination of the drug substance and its capsules, the method is simple, rapid, good stability. Methods: C_ (18) bonded silica column was used. The mobile phase consisted of acetonitrile - pH 3.0 buffer (3: 2) and pH 3.0 buffer was treated with 0.02M NaH 2 PO 4 ) And then 0.01M citric acid adjusted to pH2.97 ~ 3.04. The flow rate was 0.5 ml / min and was detected at UV 254 nm. The sample was dissolved or extracted with methanol, and finally a methanol solution (standard preparation) having a concentration of 1 mg / ml and containing an internal standard of 4-nitrobenzoic acid 0.6 mg / ml was prepared for 2 μl each and the peak height