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目的探讨肺结核患者血清中巨噬细胞相关细胞因子IL-6、IL-12、IL-23、TNF-α、IFN-γ的变化及其临床意义。方法采用酶联免疫吸附法(ELISA)检测30例活动性肺结核患者(患者);30例结核分枝杆菌感染者(感染者);30例健康人血清中的IL-6、IL-12、IL-23、TNF-α、IFN-γ分泌水平。结果 IL-6在健康人、患者者和感染组血清中的分泌水平分别为:0.43±0.03 pg/ml,0.86±0.04 pg/ml,0.46±0.02 pg/ml;IL-12在健康人、患者者和感染组血清中水平分别为:9.43±0.22 pg/ml,14.96±0.58 pg/ml,9.58±0.20 pg/ml;IL-23在健康人、患者者和感染组血清中水平分别为:269.58±28.58pg/ml,336.58±30.61 pg/ml;332.03±25.71 pg/ml;IFN-γ在健康人、患者者和感染组血清中水平分别为:90.80±2.05 pg/ml,116.15±4.96 pg/ml,114.29±3.16 pg/ml;TNF-α在健康人、患者者和感染组血清中水平分别为:13.87±3.28 pg/ml,14.06±2.14 pg/ml,13.43±3.12 pg/ml。统计分析发现:IL-6、IL-12、IL-23、IFN-γ在患者血清中的分泌水平显著高于健康对照组(p<0.05);IL-23与INF-γ在感染者血清中的分泌水平显著高于健康对照组(p<0.05);IL-6与IL-12在患者血清中的分泌水平显著感染者组(p<0.05);其余细胞因子在3类人群血清中分泌水平差别无统计学意义。结论血清中巨噬细胞相关细胞因子IL-6、IL-12、IL-23、IFN-γ在肺结核发病中具有重要意义,具有成为肺结核临床诊断依据可能。
Objective To investigate the changes of macrophage-associated cytokines IL-6, IL-12, IL-23, TNF-α and IFN-γ in patients with pulmonary tuberculosis and their clinical significance. Methods Thirty active pulmonary tuberculosis patients (patients) were detected by enzyme-linked immunosorbent assay (ELISA). Thirty cases of Mycobacterium tuberculosis infection (infected persons) and 30 healthy subjects were detected for IL-6, IL- -23, TNF-α, IFN-γ secretion levels. Results The levels of IL-6 in sera of healthy people, patients and infected patients were 0.43 ± 0.03 pg / ml, 0.86 ± 0.04 pg / ml and 0.46 ± 0.02 pg / ml, respectively. IL- The serum levels of IL-23 and healthy volunteers were respectively 9.43 ± 0.22 pg / ml, 14.96 ± 0.58 pg / ml and 9.58 ± 0.20 pg / ml. The serum levels of IL-23 were 269.58 ± 28.58pg / ml, 336.58 ± 30.61pg / ml; 332.03 ± 25.71pg / ml; The levels of IFN-γwere 90.80 ± 2.05 pg / ml and 116.15 ± 4.96 pg / ml respectively in healthy people, ml and 114.29 ± 3.16 pg / ml, respectively. The levels of TNF-α in serum of healthy people, patients and infected patients were 13.87 ± 3.28 pg / ml, 14.06 ± 2.14 pg / ml and 13.43 ± 3.12 pg / ml, respectively. Statistical analysis showed that the secretion levels of IL-6, IL-12, IL-23 and IFN-γ in patients serum were significantly higher than those in healthy controls (p <0.05). IL-23 and INF- (P <0.05). The levels of IL-6 and IL-12 in sera of patients were significantly higher than those of healthy controls (p <0.05). The levels of other cytokines in sera of three groups were significantly higher than those in healthy controls The difference was not statistically significant. Conclusion Serum macrophage-associated cytokines IL-6, IL-12, IL-23 and IFN-γ are of great significance in the pathogenesis of pulmonary tuberculosis and have the potential to become the clinical diagnosis of pulmonary tuberculosis.