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作者在建立检测庚型肝炎病毒酶免疫和ＰＣＲ技术的基础上，将抗－ＨＧＶ阳性病人血清经逆转录─巢式ＰＣＲ分离部分ＨＧＶｃＤＮＡ片段，用与ＨＧＶ基因互补的特异寡酸引物进行双脱氧核苷酸链末端终止法──ＰＣＲ直接测序。结果表明，中国大陆ＨＧＶ－３０２Ⅰ株部分ｃＤＮＡ序列与Ｌｉｎｎｅｎ等报道的ＨＧＶ美国株ｃＤ－ＮＡ序列有极高的同源性。５’端非编码区、前１区、包膜Ｅ１区、ＮＳ３区和ＮＳ５区与美国株ｃＤＮＡ序列有高达９０％以上的同源性，编码相应氨基酸的同源性达９６％。表明ＨＧＶ感染虽呈全球分布，但在进化上属遗传高度保守。
Based on the detection of hepatitis G virus (HCV) and PCR, the serum of anti-HGV-positive patients was separated by reverse transcription-nested PCR and HGV cDNA fragments were separated. Doxygenation was performed with specific oligo primers complementary to HGV gene Glycosidase chain termination method ─ ─ PCR direct sequencing. The results showed that the partial cDNA sequence of HGV-302Ⅰ in mainland China had very high homology with the cD-NA sequence of HGV in USA reported by Linnen et al. The 5 ’untranslated region, the first region, the E1 region of the envelope, the NS3 region and the NS5 region have high homology of more than 90% with the cDNA sequence of the U.S. strain, and the homology of the corresponding amino acid sequence is 96%. HGV infection showed that although the global distribution, but the genetic evolution is highly conserved.