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通过室内测定和田间观察 ,就转sck/cry1Ac双基因抗虫水稻MSB和MSA对我国主要水稻害虫二化螟和稻纵卷叶螟的抗虫效果进行了研究。室内离体植株测定结果表明 ,MSB、MSA高抗两种害虫 ,抗性水平受水稻苗龄影响。 6 5～71d苗龄水稻上害虫校正死亡率达 98.9%～ 10 0 % ,10 5～ 10 9d时抗性水平有所降低 ,但除MSB对二化螟的校正死亡率 (仍达 79.3% )降低明显 (P <0 .0 5 )外 ,MSA对二化螟及MSB、MSA对稻纵卷叶螟的校正死亡率 (92 .8%～ 98.1% )均无明显下降(P>0 .0 5 )。选取MSB进行的田间实验进一步明确 ,MSB对两种害虫有显著控虫效果 ,抗性水平与抗虫对照克螟稻 1号(含cry1Ab)无明显差异(P >0 .0 5 ) ,且略优于施药亲本明恢 86。相对于未施药明恢 86 ,MSB对两种害虫的防治效果分别为 92 .6 %、97.3% ,施药明恢 86则分别为 87.7%、89.8%。
Through the indoor determination and field observation, the insect-resistant effects of sck / cry1Ac double-gene pest-resistant rice MSB and MSA on rice stem borer and rice leaf roller were studied. The results of in vitro isolated plants showed that MSB and MSA were highly resistant to two pests and the resistance level was affected by the seedling age of rice. The corrected mortality rate of rice pests at 5 ~ 71d seedling age was 98.9% -10.0%, and the resistance level was reduced at 10 5 ~ 10 9d, except for the corrected mortality (still up to 79.3%) of the stem borer (P <0.05), the corrected mortality of MSA to the rice stem borer, Chilo suppressalis (Walker), MSB and MSA (92.8% -98.1%) was not significantly decreased (P> 0.05) 5). MSB conducted field experiments to further clarify the MSB on pest insects have significant pest control effect, the resistance level and insecticide control Kedao rice 1 (including cry1Ab) no significant difference (P> 0.05), and slightly Better than the drug parents Ming Hui 86. The control effect of MSB on the two pests was 92.6% and 97.3%, respectively, compared with that of Minghui 86, which was 87.7% and 89.8% respectively.