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目的探讨雾化吸入丹参酮ⅡA磺酸钠(STS)对脂多糖(LPS)诱导Wistar大鼠急性肺损伤(ALI)的治疗机制和临床意义。方法给大鼠尾静脉注射LPS(5mg/kg)复制ALI动物模型。随机分成4组。正常对照组(雾化吸入生理盐水,2ml.次-1.只-1,1次/12h,15min/次);LPS模型组(尾静脉注射LPS即刻开始雾化吸入生理盐水,2ml.次-1.只-1,1次/12h,15min/次);正常大鼠雾化吸入STS(正常大鼠每日雾化吸入STS,2ml.次-1.只-1,1次/12h,15min/次);STS治疗组(尾静脉注射LPS即刻开始雾化吸入STS,2ml.次-1.只-1,1次/12h,15min/次)。每组大鼠分别于2、48h和7d三个时间点各处死6只按时相观察和收集标本,进行测定和病理切片检查。测定肺湿重/肺干重、肺含水率,光镜观测肺组织病理变化,检测在实验性ALI大鼠肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)、血栓素B2(TXB2)、髓过氧化物酶(MPO)等细胞因子、炎性介质的表达。各组大鼠动脉血气分析比较。结果尾静脉注射LPS后肺湿/干重量比(W/D)(P<0.05)和血清TNF-α、IL-6、TXB2及肺组织MPO水平显著高于正常对照组(P<0.01)。而雾化吸入STS可显著缓解上述变化(P<0.01)。LPS模型组二氧化碳分压(PaO2)较对照组显著降低(P<0.01);雾化吸入STS治疗组PaO2显著升高(P<0.05),PaCO2显著低于LPS组(P<0.05)。正常大鼠雾化吸入STS组与正常对照组比较无统计学差异。结论雾化吸入STS通过降低IL-6、TNF-α释放减轻病理损害而在治疗ALI中有一定应用前景。
Objective To investigate the therapeutic mechanism and clinical significance of inhalation of sodium tanshinone Ⅱ A sulfonate (STS) on lipopolysaccharide (LPS) - induced acute lung injury (ALI) in Wistar rats. Methods LPS (5mg / kg) was injected into the tail vein of rats to replicate the animal model of ALI. Randomly divided into 4 groups. Normal control group (atomized inhalation of saline, 2ml. Times -1 only -1,1 times / 12h, 15min / times); LPS model group (LPS injection of tail vein immediately began inhalation of saline, 2ml. 1. Only -1,1 / 12h, 15min / time); normal rats inhaled STS (normal rats inhaled daily STS, 2ml. Times -1 only -1,1 / 12h, 15min / Times); STS treatment group (STPS, 2ml. Times-1, only-1, 1 times / 12h, 15min / times) Each group of rats at the time points of 2,48h and 7d were sacrificed 6 time-phase observation and collection of specimens, determination and biopsy. The lung wet weight, dry weight of lung and water content of lung were measured. Pathological changes of lung tissue were observed with light microscope. The levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) B2 (TXB2), myeloperoxidase (MPO) and other cytokines, inflammatory mediators expression. Arterial blood gas analysis and comparison in each group. Results The wet / dry weight ratio (W / D) (P <0.05) and the levels of TNF-α, IL-6, TXB2 and MPO in the lung tissue were significantly higher than those in the normal control group (P <0.01) Inhaled STS inhalation can significantly alleviate these changes (P <0.01). PaO2 in LPS model group was significantly lower than that in control group (P <0.01); PaO2 was significantly increased (P <0.05), PaCO2 was significantly lower in STS treatment group than that in LPS group (P <0.05). Normal rats inhaled STS group compared with the normal control group was no significant difference. Conclusion Inhalation of STS by atomization can reduce the pathological damage by reducing the release of IL-6 and TNF-α, and has certain application prospect in the treatment of ALI.