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本研究以辣椒胞质雄性不育系131BC5A与恢复系139D-21-3为亲本,构建了包含210个单株的F2群体,采用CAPS、SSR及SCAR等分子标记技术,对辣椒胞质雄性不育恢复基因进行遗传分析和基因定位研究.田间调查和遗传分析结果表明,F2群体的表现型中可育与不育的分离比为3:1.采用分离群体分组分析法(BSA),从F2可育群体和不育群体中各随机选取10株,构建可育和不育基因池.研究选用295对引物在亲本间进行多态性筛选,其中43对引物在亲本间表现出多态.通过进一步分析43对引物在基因池间的多态性,筛选出Rf基因连锁标记14个.然后对F2群体的210个单株进行连锁分析,最后将恢复基因定位在SSR标记pep43和pep20之间,约3.9 cM(或498.6 kb)的区间内,与两个标记的遗传距离分别为1.3 cM与2.6 cM.本研究为Rf基因的精细定位和克隆奠定了良好基础,也为辣椒雄性不育恢复系的分子标记辅助选择育种提供了理论参考.“,”With 210 individuals of F2 segregation population from the crossing of CMS line 131BC5A with restorer line 139D-21-3 as materials,the heredity and molecular genetic mapping of the fertility restorer gene were explored by CAPS,SSR and SCAR techniques in this study.We found that F2 population may be divided into fertile individuals and sterile individuals by field investigation and genetic analysis,and their proportion fit for 3:1.Constructing fertile and sterile gene pools by taking bulked segregant analysis (BSA) which select 10 individuals from F2 fertile population and sterile population randomly and mix their DNA respectively.A total of 295 pairs of primers were used to detect polymorphism between the two parents,and only 43 polymorphic markers were detected.The 43 pairs of primers were further used to screen for polymorphisms between the fertile and sterile DNA pools,and we found 14 markers which have polymorphism between two gene pools was linked to Rf gene.By analyzing 210 F2 plants using the six polymorphic markers,Rfwas mapped in a 3.9 cM (or 498.6 kb) interval between SSR markers pep43 and pep20,with genetic distances of 1.3 cM and 2.6 cM to the two markers,respectively.The results in this study will be of great benefit to fine mapping and gene cloning for the Rf gene and lay a good foundation for MAS of restoring line of pepper male sterile.