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以‘秦美’猕猴桃无菌苗叶片为外植体,通过不定芽诱导、继代增殖和生根培养基的筛选,建立高频直接再生体系.结果表明,叶片出芽最佳培养基为MS+5.0mg/L 6-BA+0.2mg/L NAA,40d出芽率达100%,每个叶盘平均出芽7.4个;不定芽继代增殖最佳培养基为MS+5.0mg/L 6-BA+0.2mg/L NAA+0.1mg/L GA3,每30d继代1次,1~5代平均繁殖系数达6.43;不定芽生根最佳培养基为1/2 MS+0.7 mg/L IBA,30d生根率为91.11%.在土壤营养钵中,试管苗30d移栽成活率达92.47%.本试验成功建立了‘秦美’猕猴桃的叶片高频直接再生体系,为其遗传转化奠定了基础.
The explants of ’Qin Mei’ aseptic seedlings were used as explants to establish direct high-frequency regeneration system by adventitious buds induction, subculture and rooting medium selection.The results showed that the optimum culture medium for leaf budding was MS + 5.0 The budding rate reached 100% on the 40th day with the medium of mg / L 6-BA + 0.2mg / L NAA. The average number of buds per leaf disc was 7.4. The best medium for adventitious buds proliferation was MS + 5.0mg / L 6-BA + 0.2 The average reproductive coefficient of 1 ~ 5 generations reached 6.43. The optimal medium for adventitious buds rooting was 1/2 MS + 0.7 mg / L IBA, the rooting rate was 30 days Was 91.11% .There was a 92.47% survival rate of test tube seedlings transplanted in soil nutrition bowl in 30 days.In this experiment, direct high-frequency regeneration system of ’Qinmei’ kiwifruit was established, which laid the foundation for its genetic transformation.