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目的建立同时测定猫豆中左旋多巴(2)及其3种衍生物含量的高效液相色谱法。方法从猫豆中分离获得3-羧基-6,7-二羟基-1,2,3,4-四氢异喹啉(1)、1-甲基-3-羧基-6,7-二羟基-1,2,3,4-四氢异喹啉(3)、1,1二甲基-3-羧基-6,7-二羟基-1,2,3,4-四氢异喹啉(4)等3种成分;含量测定采用Cosmosil C18-PAQ色谱柱(4.6 mm×250 mm,5?m),流动相1%乙酸等度洗脱,流速0.5 m L·min?1,柱温30℃,检测波长280 nm。结果左旋多巴(2)和3个化合物色谱分离良好,分别在0.298 4~5.968?g(1),6.554~131.1?g(2),0.262 6~5.252?g(3),0.256 8~5.136?g(4)线性关系良好(r>0.999),平均加样回收率依次为97.7%,99.1%,98.7%,97.9%。结论本方法简便、准确,可用于同时测定猫豆中的左旋多巴及其四氢异喹啉类衍生物含量,为其质量控制方法提供参考。
Objective To establish a HPLC method for simultaneous determination of levodopa (2) and its derivatives in catfish. Methods The 3-carboxy-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline (1), 1-methyl-3-carboxy-6,7-dihydroxy Tetrahydroisoquinoline (3), 1,1-dimethyl-3-carboxy-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline ( 4). The determination was performed on a Cosmosil C18-PAQ column (4.6 mm × 250 mm, 5 μm) with a mobile phase of 1% acetic acid at a flow rate of 0.5 mL · min -1 and a column temperature of 30 ℃, detection wavelength 280 nm. Results The chromatographic separation of levodopa (2) and 3 compounds was good, with a linear range from 0.298 4 to 5.968 μg (1), 6.554-131.1 μg (2), 0.262 6-5.252 μg (3), 0.256 8-5.136 g (4) had good linearity (r> 0.999). The average recoveries were 97.7%, 99.1%, 98.7% and 97.9%, respectively. Conclusion The method is simple and accurate and can be used for the simultaneous determination of levodopa and its tetrahydroisoquinoline derivatives in cat bean and provide references for its quality control.