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Background. We investigated the possible roles of the interleukin(IL)-15 and IL-15 receptor (IL-15R) system in a heightened state of B-cell activation and differentiation in intestinal mucosa with inflammatory bowel disease (IBD).Meth ods. The expression of IL-15 and IL-15RαmRNA and protein in inflamed colonic mucosal tissues with IBD, and in control tissues was examined by reverse transcr iptase-polymerase chain reaction and immunohistological methods. The effects of recombinant(r)IL-15 on the expression of IL-15Rαon lamina propria B cells an d the production of immunoglobulin G (IgG) were analyzed in vitro, using lamina propria mononuclear cells (LPMCs)isolated from control tissues. Results. The int ensity of IL-15 and IL-15RαmRNA was greater in the mucosal tissues of patient s with IBD, especially in those of patients with ulcerative colitis (UC), than i n control tissues. Compared to control tissues, mononuclear cells positive for I L-15Rαprotein were observed in greater proportions in tissue sections from pat ients with IBD, especially in those from patients with UC, where IL-15Rαprotei n was localized to CD20-positive B cells to a significant degree. There were in creases in the proportions of IL-15Rα-positive B cells and IgG-producing cel ls in rIL-15-or rCD40L-stimulated cultures of LPMCs, with stimulatory effects being greater in the presence of their combination.Conclusions. These data sugg est that the IL-15 and IL-15R system may play important roles in the activatio n and differentiation of lamina propria B cells in patients with IBD, especially in those with UC.
Background. We investigated the possible roles of the interleukin (IL) -15 and IL-15 receptor (IL-15R) system in a heightened state of B-cell activation and differentiation in intestinal mucosa with inflammatory bowel disease (IBD). Meth ods . The expression of IL-15 and IL-15Rα mRNA and protein in inflamed colonic mucosal tissues with IBD, and in control tissues was examined by reverse transcr iptase-polymerase chain reaction and immunohistological methods. The effects of recombinant (r) IL-15 on The expression of IL-15Rα lamina propria B cells an d the production of immunoglobulin G (IgG) were analyzed in vitro, using lamina propria mononuclear cells (LPMCs) isolated from control tissues. Results. The int ensity of IL-15 and IL- Compared to those of control tissues, mononuclear cells positive for I L-15Rαprotein were observed in greater propor- tions tions in tissue sections from pat ients with IBD, especially in those from patients with UC, where IL-15Rαprotein was localized to CD20-positive B cells to a significant degree. There were in creases in the proportions of IL-15Rα-positive B cells and IgG-producing cel ls in rIL-15-or rCD40L-stimulated cultures of LPMCs, with stimulatory effects being greater in the presence of their combination. Conclusions. These data sugg est that the IL-15 and IL-15R system may play important roles in the activatio n and differentiation of lamina propria B cells in patients with IBD, especially in those with UC.