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用海岛棉茎尖作为基因枪转化的靶材料,建立了可重复的海岛棉转化系统。将海岛棉茎尖分生组织经过3～6周的诱导、继代培养生长后,可以形成足够量的再生植株。用含有Npt-Ⅱ基因和蜘蛛丝蛋白基因的质粒轰击新疆海岛棉4个品种茎尖分生组织,在含有70～100mg/l卡那霉素的培养基上筛选、预再生、再生及生根培养,获得的24株抗卡那霉素转基因再生植株。所获得的转基因植株经卡那霉素筛选,再生植株总DNA提取,Southern点杂交分子检测,初步证明有2株转化植株目的基因已整合到海岛棉基因组中,同时也已得到种子。研究为通过植物基因工程的方法以期改良棉花纤维品质提供了一条新的途径。
With island tip of the island cotton as the target material for gene gun transformation, a repeatable island island cotton transformation system was established. The island meristematic meristem after 3 to 6 weeks of induction, subculture and growth, you can form a sufficient amount of regenerated plants. The shoot apical meristem of 4 cultivars of G. barbadense was bombarded with plasmid containing Npt-Ⅱ gene and spider silk protein gene, and then selected on medium containing 70-100 mg / l kanamycin, pre-regeneration, regeneration and rooting , 24 anti-kanamycin transgenic plants were obtained. The obtained transgenic plants were screened by kanamycin, total DNA extracted from the regenerated plants and Southern blot hybridization. It was initially proved that the target genes of two transformed plants had been integrated into the island cotton genome and the seeds had also been obtained. The research provides a new way to improve cotton fiber quality through plant genetic engineering.