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【目的】观察清燥救肺汤对小鼠结肠癌CT26细胞增殖相关蛋白原癌基因c-myc,表皮细胞生长因子(EGF)及侵袭相关蛋白细胞间黏附分子1(ICAM-1)、基质金属蛋白酶2(MMP2),转化生长因子α(TGF-α)表达的影响。【方法】将50只雄性BALB/c小鼠随机分为模型组,化疗组,清燥救肺汤高、中、低剂量组,每组10只。右腋下注射CT26细胞建立结肠癌小鼠模型,清燥救肺汤治疗组以15.2、7.6、3.8 g·kg~(-1)·d~(-1)剂量于造模前两周开始灌胃给药,化疗组给予25 mg·kg~(-1)剂量5-氟尿嘧啶(5-FU)腹腔注射,模型组以等体积生理盐水灌胃给药。2周后处死各组小鼠并取瘤组织,称质量计算抑瘤率,以Western-blot法检测EGF,TGF-α,c-myc,ICAM-1及MMP2蛋白表达。【结果】清燥救肺汤高、中剂量组EGF,ICAM-1,MMP2及TGF-α蛋白表达显著降低,与模型组比较,差异均有统计学意义(P<0.05或P<0.01);清燥救肺汤高、中、低剂量组c-myc蛋白表达显著降低,与模型组比较,差异有统计学意义(P<0.05)。【结论】清燥救肺汤可能通过降低EGF、TGF-α及c-myc、ICAM-1、MMP2蛋白表达,发挥抑制荷CT26小鼠结肠癌细胞增殖侵袭的功效。
【Objective】 To observe the effects of Qingzaojiufei decoction on the expression of c-myc, epidermal growth factor (EGF) and intercellular adhesion molecule-1 (ICAM-1) in mouse colon carcinoma CT26 cells, Protease 2 (MMP2), transforming growth factor alpha (TGF-alpha) expression. 【Methods】 Fifty male BALB / c mice were randomly divided into model group, chemotherapy group, Qingzaojianfei Decoction high, medium and low dose group, 10 rats in each group. The right armpit injection of CT26 cells to establish a mouse model of colon cancer, Qingzao Yifei Tang treatment group at 15.2,7.6,3.8 g · kg -1 d -1 dose two weeks before the start of modeling The rats in the chemotherapy group were given intraperitoneal injection of 5-fluorouracil (5-FU) at a dose of 25 mg · kg -1. The model group was given intragastric administration of normal saline. After 2 weeks, the mice in each group were killed and the tumor tissues were removed. The tumor inhibition rate was calculated by mass and the expression of EGF, TGF-α, c-myc, ICAM-1 and MMP2 were detected by Western-blot. 【Results】 The results showed that the expression of EGF, ICAM-1, MMP2 and TGF-α in Qingpi Yifei Decoction group were significantly lower than those in model group (P <0.05 or P <0.01); The expression of c-myc protein of Qingzaojiu Decoction high, medium and low dose groups was significantly lower than that of model group (P <0.05). 【Conclusion】 Qingzao Yifei Decoction may inhibit the proliferation and invasion of CT26 mouse colon carcinoma cells by reducing the expression of EGF, TGF-α, c-myc, ICAM-1 and MMP2.