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目的探讨含BPVL1/HPV16E7嵌合型乳头瘤病毒样颗粒(VLPs)在动物小鼠体内的免疫学特性。方法将HPV16E7基因分为(a)、(b)、(c)3段,与BPVL1连接后表达的BPVL1/HPV16E7(a)、(b)、(c)嵌合型VLP分别免疫小鼠C57BL/6J,对其淋巴结淋巴细胞进行细胞毒性T淋巴细胞反应(CTL)分析。结果接受嵌合型VLPBPVL1/HPV16E7(b)免疫的小鼠淋巴结淋巴细胞可以引发很强的对C-2细胞(HPV16E7aa47-59转染的EL-4细胞)的特异性CTL反应,但不能对EL-4细胞产生细胞毒性作用。结论含BPVL1/HPV16E7嵌合型VLP作为抗原输送系统致敏小鼠T淋巴细胞并引发抗原特异性CTL反应,可能为HPV疫苗的设计提供一个新手段。
Objective To investigate the immunological characteristics of BPVL1 / HPV16E7 chimeric papillomavirus-like particles (VLPs) in animal mice. Methods The HPV16E7 gene was subcloned into three segments of (a), (b) and (c). The BPVL1 / HPV16E7 (a), (b) and (c) 6J, lymphocytic lymphocyte cytotoxic T lymphocyte reaction (CTL) analysis. Results Mice lymph node lymphocytes immunized with chimeric VLPBPVL1 / HPV16E7 (b) elicited a strong CTL response to C-2 cells (EL-4 cells transfected with HPV16 E7aa47-59) but not EL -4 cells produce cytotoxic effects. Conclusion The chimeric VLP containing BPVL1 / HPV16E7 as antigen delivery system can sensitize mouse T lymphocytes and induce antigen-specific CTL responses, which may provide a new approach for the design of HPV vaccines.