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研究人参皂苷Rg1对皮质酮介导的星形胶质细胞损伤的保护作用,并对其作用机制进行初步探索。分离培养原代海马和前额叶皮质区星形胶质细胞,给予皮质酮(corticosterone,CORT)模拟应激条件,采用Western blot方法检测Rg1对Cx43磷酸化水平的影响;利用Cell Counting Kit(CCK8)方法检测Rg1对细胞生存率的影响考察及蛋白酶抑制剂是否影响Rg1对细胞生存率的保护作用。结果显示,人参皂苷Rg1显著降低CORT介导的Cx43磷酸化水平升高。与CORT(200μmol·L-1)组相比,人参皂苷Rg1(10μmol·L-1)可显著增加海马和前额叶皮质星形胶质细胞的生存率;人参皂苷Rg1的保护作用在海马星形胶质细胞可被蛋白激酶Src抑制剂PP2、p38抑制剂SB203580及Akt的抑制剂BAY1125976抑制,而在前额叶皮质星形胶质细胞仅可被Src抑制剂PP2和Akt的抑制剂BAY1125976抑制。结果表明,人参皂苷Rg1可显著减轻CORT所致的星形胶质细胞缝隙连接通道蛋白Cx43活性的降低,并通过激活Src、p38与Akt信号通路发挥抗皮质酮损伤的作用,这种作用在海马和前额叶皮质存在脑区差异性。
To study the protective effect of ginsenoside Rg1 on corticosterone-induced injury of astrocytes, and to explore its mechanism. The primary hippocampus and prefrontal cortex astrocytes were isolated and cultured, and the cortisol (CORT) was used to simulate the stress conditions. The effect of Rg1 on Cx43 phosphorylation was detected by Western blot. Cell Counting Kit (CCK8) Methods The effect of Rg1 on the cell survival rate and the protective effect of protease inhibitor on the cell survival rate were investigated. The results showed that ginsenoside Rg1 significantly reduced CORT-mediated Cx43 phosphorylation. Compared with CORT (200μmol·L-1) group, ginsenoside Rg1 (10μmol·L-1) significantly increased the survival rate of hippocampus and prefrontal cortex astrocytes; ginsenoside Rg1 protective effect in the hippocampus Glial cells were inhibited by protein kinase Src inhibitor PP2, p38 inhibitor SB203580 and Akt inhibitor BAY1125976, whereas prefrontal cortex astrocytes were only inhibited by Src inhibitor PP2 and Akt inhibitor BAY1125976. The results showed that ginsenoside Rg1 could significantly reduce the CORT-induced decrease of Cx43 activity in astrocytes and activate corticosterone damage by activating Src, p38 and Akt signaling pathway in hippocampus And prefrontal cortex exist brain area difference.