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目的评价纸片扩散法(K-B法)检测淋病奈瑟菌对两种第三代头孢菌素类药物敏感性的准确率。方法在广西部分地区性病科门诊收集了262株淋病奈瑟菌菌株,采用K-B法和琼脂稀释法检测淋病奈瑟菌对头孢曲松和头孢克肟两种药物的敏感性。结果 K-B法和琼脂稀释法对头孢曲松敏感菌株的检测率分别为93.1%和100%,对头孢克肟敏感菌株的检测率分别为90.8%和99.2%。K-B法检测了244株菌株对头孢曲松药物的敏感性,与琼脂稀释法检测的最小抑菌浓度结果一致,发现18株敏感菌株被误判为非敏感菌株;K-B法检测出238株菌株对头孢克肟药物敏感,其中236株菌株与琼脂稀释法检测最小抑菌浓度结果一致,24株敏感菌株误判为非敏感菌株,有2株非敏感菌株被误判为敏感菌株。K-B法对两种头孢菌素类药物敏感菌株的检测率与琼脂稀释法检测结果差异有统计学意义。结论在应用于临床时应注意其应用范围,如遇到K-B法敏感结果时可直接指导临床用药,但当K-B法结果出现非敏感时,应采用琼脂稀释法来最终判定其敏感性。
Objective To evaluate the accuracy of the K-B method in detecting the sensitivity of Neisseria gonorrhoeae to two third-generation cephalosporins. Methods A total of 262 strains of Neisseria gonorrhoeae were collected from some endemic clinics in Guangxi. The sensitivity of Neisseria gonorrhoeae to ceftriaxone and cefixime was tested by K-B method and agar dilution method. Results The detection rates of ceftriaxone sensitive strains by K-B method and agar dilution method were 93.1% and 100%, respectively. The detection rates of cefixime sensitive strains were 90.8% and 99.2% respectively. KB method detected 244 strains of ceftriaxone drug sensitivity, and agar dilution method to detect the minimum inhibitory concentration results and found 18 sensitive strains were misjudged as non-sensitive strains; KB method to detect 238 strains of Among them, 236 strains were agar dilution method to detect the minimum inhibitory concentration (MIC). The results of 24 sensitive strains were misjudged as non-sensitive strains, and 2 non-sensitive strains were misjudged as sensitive strains. K-B method for two cephalosporin-sensitive strains of the detection rate and agar dilution test results were statistically significant. Conclusions The clinical application should pay attention to its application, such as K-B method in the face of sensitive results can directly guide clinical medication, but when the results of K-B method is not sensitive, the agar dilution method should be used to determine its sensitivity.