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用成虫抗原免疫免血清筛选构建于表达载体λgt11的日本血吸虫成虫cDNA文库，再以多聚酶链反应（PCR）技术鉴定免疫筛选的阳性克隆。结果，对cDNA表达库中随机选取的约6.2×104个噬菌斑进行了筛选．初筛时共挑出52个可能的阳性斑，经两次复筛后，有25个噬菌仍呈阳性反应；以阳性噬菌斑DNA为模板，经PCR扩增均可获得一定大小的片段，从564bp到1200bp不等。这样能快速大量地筛选cDNA文库，便于高效地获得有价值的重组抗原基因克隆。
Immunization with serum free of adult antigens was used to screen the cDNA library of adult Schistosoma japonicum cDNA that was constructed on expression vector λgt11. The positive clones were screened by polymerase chain reaction (PCR). As a result, about 6.2 × 10 4 plaques randomly selected from the cDNA expression library were screened. A total of 52 possible positive plaques were picked out during initial screening. After two repeated screening, 25 phages were still positive. Positive plaque DNA was used as a template to obtain fragments of certain size , Ranging from 564bp to 1200bp. This allows for rapid and extensive screening of cDNA libraries for efficient and efficient access to valuable recombinant antigen gene clones.