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目的探讨结直肠癌组织中CD44v6和β-catenin蛋白表达及其意义。方法采用微波-EliVisionTM免疫组化染色方法检测60例结直肠癌组织中CD44v6和β-catenin的表达情况,并分析其与临床病理参数尤其与结直肠癌浸润转移的关系。结果阳性表达物质CD44v6主要在细胞膜和细胞质,β-catenin主要在细胞质。60例结直肠癌CD44v6和β-catenin蛋白阳性表达率分别为71.7%和81.7%。两者均与肿瘤的临床TNM分期、浸润程度、淋巴结转移及术后复发有关;上述参数各分组间差异有统计学意义(P<0.05)。结论联合检测CD44v6-和β-catenin蛋白表达对了解结直肠癌的生物学行为和判断患者预后有一定的实用价值。
Objective To investigate the expression of CD44v6 and β-catenin in colorectal cancer and its significance. Methods The expression of CD44v6 and β-catenin in 60 cases of colorectal cancer were detected by microwave-EliVisionTM immunohistochemical staining. The relationship between CD44v6 and β-catenin and the clinicopathological parameters was analyzed, especially the correlation with the invasion and metastasis of colorectal cancer. Results The positive expression of CD44v6 mainly in the cell membrane and cytoplasm, β-catenin mainly in the cytoplasm. The positive rates of CD44v6 and β-catenin in 60 cases of colorectal cancer were 71.7% and 81.7% respectively. Both of them were related to clinical TNM staging, infiltration degree, lymph node metastasis and postoperative recurrence. There was significant difference among the above parameters (P <0.05). Conclusions Combined detection of CD44v6- and β-catenin protein expression has certain practical value in understanding the biological behavior of colorectal cancer and judging the prognosis of patients.