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作者采用原代培养大鼠肝细胞研究黄芩有效成分的护肝作用，并对其中含量较多的结晶单体进行结构鉴定。结果表明：黄芩的乙酸乙酯萃取物和正丁醇萃取物（１．０～２．５ｍｇ／ｍｌ）均可使四氯化碳致伤的肝细胞培养液中ＡＬＴ活性显著降低（Ｐ＜０．００１）。从黄芩乙酸乙酯萃取物分离出来的晶Ⅱ、Ⅲ、Ⅳ在所试浓度下均有显著护肝作用；晶Ⅰ则主要为直接抑制酶活性作用。经理化鉴别和光谱分析，晶Ⅰ、Ⅱ分别为汉黄芩素和黄芩素。
The authors used the primary cultured rat hepatocytes to study the hepatoprotective effect of the active ingredients of Radix Astragali, and identified the structure of the crystalline monomers that contained more. The results showed that the ethyl acetate extract and n-butanol extract (1.0-2.5 mg/ml) of Astragalus membranaceus could significantly reduce the ALT activity in the carbon tetrachloride-induced hepatocyte culture medium (P<0. 001). The crystals II, III, and IV isolated from the ethyl acetate extract of Astragalus membranaceus had a significant hepatoprotective effect at the concentration tested; the crystal I was mainly to directly inhibit the activity of the enzyme. Managerial identification and spectral analysis. Crystals I and II were wogonin and baicalein, respectively.