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作者发现,人红细胞经溴化2-氨基乙基异硫脲(2-aminoethylisothiouronium bromide,AET)处理后,Kell血型抗原被特异性灭活,而其它血型抗原不受影响。用5N NaOH调节AET6％水溶液的pH到8.0(临用前配制)。1容积洗涤后的压积红细胞与4容积的上述AET溶液混合,37℃培育20分钟,然后用pH7.0磷酸盐缓冲液洗涤,直到上清液变清(4～6次),冷藏并在24小时内使用。用IgG Kell抗血清作间接抗球蛋白试验,对照研究经AET处理和不经AET处理的红细胞,发现
The authors found that the Kell blood group antigen was specifically inactivated after human erythrocytes were treated with 2-aminoethylisothiouronium bromide (AET), while the other blood group antigens were not affected. The pH of the AET6% aqueous solution was adjusted to 8.0 with 5N NaOH (prepared just before use). After 1 volume wash, the packed red blood cells were mixed with 4 volumes of the above AET solution, incubated at 37 ° C for 20 minutes and then washed with pH 7.0 phosphate buffer until the supernatant became clear (4 to 6 times) Use within 24 hours. Indirect antiglobulin test with IgG Kell antiserum, control study of AET-treated and non-AET-treated erythrocytes, found