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目的:探讨TGFβ1对人单核细胞来源DCs活性的影响。方法:在诱导DCs成熟时分别加入TGFβ1、IL2+TGFβ1、CD40L+TGFβ1以及TNFα+TGFβ1,比较不同细胞因子对DCs的细胞表面标志CD50、CD83、CD80、CD1α、CD86、HLA DR和CCR7表达的影响;对DCs分泌IL12水平的作用以及DCs活化自体T细胞能力的变化。结果:TGFβ1组DCs表面标志CD83、CD80、CD86、HLA DR、CCR7均明显低于其它各组(P<0.05);IL12的分泌水平低于其它各组(P<0.05);激发自体T细胞能力亦较弱(P<0.05);与其余各组比较,在第10天时TNFα组DCs表面分子表达较成熟,活化T细胞能力亦较强(P<0.05)。结论:TGFβ1对DCs的成熟以及免疫活性具有明显的抑制作用,而TNFα能够有效逆转TGFβ1的作用。
Objective: To investigate the effect of TGFβ1 on the activity of human monocyte-derived DCs. Methods: TGFβ1, IL2 + TGFβ1, CD40L + TGFβ1 and TNFα + TGFβ1 were added to induce DCs maturation, and the effects of different cytokines on the expression of cell surface markers CD50, CD83, CD80, CD1α, CD86, HLA DR and CCR7 were compared ; The role of DCs in the secretion of IL12 and the ability of DCs to activate autologous T cells. Results: The surface markers of CD83, CD80, CD86, HLA DR and CCR7 in TGFβ1 group were significantly lower than those in other groups (P <0.05). The secretion of IL12 was lower than other groups (P <0.05) (P <0.05). Compared with the other groups, the expression of DCs in TNFα group was more mature and activated T cells on the 10th day (P <0.05). Conclusion: TGFβ1 can significantly inhibit the maturation and immunologic activity of DCs, while TNFα can effectively reverse the effect of TGFβ1.