论文部分内容阅读
本文利用印迹杂交技术对中国华北地区黑线姬鼠Ａｐｏｄｅｍｕｓａｇｒａｒｉｕｓｐａｌｉｄｉｏｒ和韩国黑线姬鼠Ａ．ａｇｒａｒｉｕｓｃｏｒｅａｅ共１０７号标本的线粒体ＤＮＡ（ｍｉｔｏｃｈｏｎｄｒｉａｌＤＮＡ，ｍｔＤ－ＮＡ），通过８种限制性内切酶的消化，进行了限制性片段的分析。共检出３５种限制性片段和１２种单倍体类型。１２种单倍型在平均离散度为１．０１％时聚合为两个亚群：一个亚群为黑线姬鼠华北亚种，由采自中国４个不同地区的５１号标本的４种单倍型所组成；另一个亚群为黑线姬鼠朝鲜亚种，由采自韩国４个不同地区的５６号标本的８种单倍型所组成。黑线姬鼠华北亚种和朝鲜亚种在ｍｔＤＮＡ表型上表现出一定差异，这在分子水平上确立了两亚种的分类地位。为了进一步澄清黑线姬鼠种下分类的混乱，很有必要对中国其他地区的标本进行该项研究
In this paper, Apodemus agrariuspalidior Apodemus agrariuspalidior and Apodemus agrarius in North China were studied by using imprinted hybridization technique. agrariuscoreae a total of 107 specimens of mitochondrial DNA (mitochondrial DNA, mtD-NA), eight kinds of restriction endonuclease digestion, restriction fragment analysis. A total of 35 restriction fragments and 12 haplotypes were detected. Twelve haplotypes converged into two subgroups with an averaged degree of dispersion of 1.01%: one subpopulation was a northern subfamily of Apodemus agrarius, consisting of four species from 51 specimens in four different regions of China Fold type; the other subpopulation is Apodemus kunthus North Asian subspecies, consisting of 8 haplotypes from 56 specimens from 4 different areas in South Korea. The northern line Apodemus agrarius North and North Asian subspecies mtDNA phenotype showed some differences, which established the molecular level of the two subspecies taxonomic status. In order to further clarify the confusion of Apodemus agrarius species, it is necessary to carry out the study on specimens from other parts of China