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目的研究选择性脊神经后根切断术(SPR)缓解痉挛型脑瘫动物泌涎症状的机理。方法建立SPR动物模型,以假手术组和正常对照组进行对照。免疫组化法检测c-fos在模型大鼠腰3-6脊髓内及脑干网状结构抑制区、易化区的表达。结果SPR造成的损伤引起SPR组脊髓后角及脑干网状结构易化区c-fos的显著性表达,与假手术组相比具有显著性差别(P<0.05)。结论c-fos表达情况的检测说明L3-6脊神经后根的传入纤维和脑干网状结构易化区存在着相关功能联系和神经通路联系,SPR术对脊神经后根的切断调整了脑干网状结构易化区的活性,通过相关脑神经核,调整咽喉肌的紧张性使流涎症状缓解。
Objective To study the mechanism of selective spinal nerve root posterior rhizotomy (SPR) to alleviate the symptoms of spastic paralysis in animals with spastic cerebral palsy. Methods Animal models of SPR were established and compared with sham operation group and normal control group. Immunohistochemistry was used to detect the expression of c-fos in the inhibition zone and the easiness zone in the lumbar 3-6 spinal cord and brainstem reticular formation in model rats. Results The injury caused by SPR caused significant expression of c-fos in spinal cord posterior horn and brainstem reticular structure-facilitated region in SPR group, which was significantly different from that in sham operation group (P <0.05). Conclusions The detection of c-fos expression shows that the afferent fibers in the posterior root of L3-6 posterior root and the facilitation of reticular formation in the brainstem have related functional connections and neural pathway connections. The SPR regulates the brainstem of the dorsal root of the dorsal root ganglion Reorganization of the network structure of the activity, through the relevant brain nucleus, adjust the tension of the throat muscles to salivating symptoms ease.