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目的:探讨大黄酸对内毒素血症小鼠的保护作用。方法:将45只雄性昆明小鼠随机分为空白组、模型组和大黄酸组,每组15只。空白组腹腔消毒注射生理盐水(10mg/kg),其余各组腹腔消毒注射LPS(10mg/kg)。治疗组于攻毒前0.5h、攻毒后1h和取血前0.5h给予大黄酸1.4mg.10g-1.d-1灌胃,空白组和模型同时按比例给予等量生理盐水。8 h后在无菌条件下采集心室血0.5mL进行血浆ET检测,取部分肝脏进行免疫组化和生化检测,取肝进行组织学观察。结果:大黄酸组对小鼠内毒素、TNF-α、IL-1β、IL-10、MDA、SOD和肝组织病理炎症等改善优于其他组。结论:大黄酸对小鼠内毒素血症有显著的保护作用,其途径可能通过改善微循环,改善肝脏的能量代谢,减轻肝脏的脂质过氧化反应,降低机体的内毒素水平,从而减少炎性因子的产生,阻断内毒素血症的生物学效应。
Objective: To investigate the protective effect of rhein on endotoxemia in mice. Methods: Forty five male Kunming mice were randomly divided into blank group, model group and rhein group, with 15 rats in each group. The rats in the blank group were injected intraperitoneally with normal saline (10mg / kg), and the other groups were injected intraperitoneally with LPS (10mg / kg). The rats in the treatment group were administered 0.5 mg rhBF1-g-1.d-1 0.5 h before challenge, 1 h after challenge and 0.5 h before blood administration. The rats in the blank group and the model were given the same amount of normal saline at the same time. After 8 h, 0.5 mL of ventricular blood was collected under aseptic conditions for plasma ET detection. Some of the liver was harvested for immunohistochemistry and biochemical detection, and the liver was taken for histological observation. Results: Rhein group was superior to other groups in improving the endotoxin, TNF-α, IL-1β, IL-10, MDA, SOD and hepatic histopathological inflammation in mice. Conclusion: Rhein has a significant protective effect on endotoxemia in mice, which may reduce the endotoxin level in the liver, improve the microcirculation, improve the energy metabolism of the liver, reduce the lipid peroxidation in the liver, and reduce inflammation The production of sexual factors, blocking the biological effects of endotoxemia.