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目的分析乌鲁木齐腹泻婴幼儿轮状病毒(Rotavirus,RV)感染流行情况及其基因型特征。方法将2007年1~12月收集于乌鲁木齐市第一人民医院(儿童医院)的3 662例5岁以下门诊和住院腹泻患儿粪便标本,采用胶体金法和ELISA法检测RV,并随机选取部分RV阳性样本进行G/P基因分型。结果 3 662例样本中检出RV阳性1 470例,检出率为40.1%。对其中142例阳性样本(约10%)进行RV PCR基因分型,其中121例婴幼儿阳性分型结果显示:G1型比例最高占36.4%,其次为G3型(20.7%)、G2型(15.7%)和G9型(13.2%),G型混合感染占6.6%,G未能分型9例(7.4%)。P型分型以P[8]为主,占60.3%,其次为P[4](18.2%)和P[6](5.8%),P混合感染占15.7%。最主要的G/P组合为G1P[8](28.1%,34/121),其次是G3P[8](19.8%,24/121)、G2P[4](14.9%,18/121)和G9P[8](8.3%,10/121),并检测到不常见组合基因型如G1P[6]、G1P[4]、G9P[4]和G9P[6]等。21例新生儿(≤28 d)阳性样本的分型结果为:G型以G9为主,占42.9%(9例),其次是G1(4例),G3(3例),G2(1例),G混合感染3例,未能分型1例。P型以P[6]为主,占47.6%(10例),其次是P[8]及Pmixed各5例,P[4]1例。G/P基因型组合以G9P[6]为主(6例),其次G1P[8]、G3P[8]和G1P[6]各2例,G2P[4]和G9P[8]各1例。结论本年度轮状病毒流行基因型复杂多样。婴幼儿RV感染以G1P[8]为主,新生儿RV感染则以G9P[6]为主。需警惕婴幼儿RV株与新生儿RV株的交叉混合感染而致新流行株的产生。
Objective To analyze the prevalence of Rotavirus (RV) infection in Urumqi diarrhea and its genotypes. Methods From January to December 2007, 3 662 urine samples from outpatients and inpatients with diarrhea were collected from Urumqi First People’s Hospital (Children’s Hospital). Colloidal gold and ELISA methods were used to detect RV, and randomly selected part RV positive samples for G / P genotyping. Results A total of 1 470 RV positive samples were detected in 3 662 samples and the detection rate was 40.1%. RV PCR genotyping was performed on 142 positive samples (about 10%). The positive typing of 121 infants and young children showed that the highest proportion of G1 type was 36.4%, followed by G3 type (20.7%), G2 type (15.7 %) And G9 (13.2%), G type mixed infection accounted for 6.6%, G failed to type 9 (7.4%). P type was mainly P [8], accounting for 60.3%, followed by P [4] (18.2%) and P [6] (5.8%), P mixed infection accounted for 15.7%. The most important G / P combinations were G1P [8] (28.1%, 34/121) followed by G3P [8] (19.8%, 24/121), G2P [4] (14.9%, 18/121) and G9P [8] (8.3%, 10/121). Uncommon genotypes such as G1P [6], G1P [4], G9P [4] and G9P [6] were also detected. The positive results of 21 newborns (≤28 days) were G9, which accounted for 42.9% (9 cases), followed by G1 (4 cases), G3 (3 cases) and G2 ), G mixed infection in 3 cases, failed to type in 1 case. P type was predominantly P [6], accounting for 47.6% (10 cases), followed by P [8] and Pmixed in 5 cases, P [4] in 1 case. The G / P genotype was predominant in G9P [6] (6 cases), followed by G1P [8], G3P [8] and G1P [6] in 2 cases, G2P [4] and G9P [8]. Conclusion The genotypes of rotavirus in this year are complex and diverse. RV infection in infants and young children to G1P [8], neonatal RV infection is mainly G9P [6]. Need to be alert to infants and young children RV strains and neonatal RV strains caused by cross-infection caused by the emergence of new strains.