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目的制备两性霉素B聚乳酸普鲁兰(PLA-pullulan)纳米胶束,并进行体外评价。方法以新型两亲性高分子PLA-pullulan为载体材料,采用薄膜水化法制备两性霉素B纳米胶束,并分别采用透射电镜、激光粒径仪和zeta电位分析仪对胶束的形态、粒径和表面电位进行表征,测定其包封率、载药量和体外释放动力学,考察其药物分散状态和溶血毒性。结果所制备的两性霉素B聚乳酸普鲁兰纳米胶束溶液外观透明,电镜下呈圆整的球形,平均粒径为152.1 nm,包封率为71.1%,载药量达10.9%,24 h的累积释放量为10.01%,溶血毒性低于两性霉素B去氧胆酸钠胶束。结论聚乳酸普鲁兰胶束是两性霉素B潜在的优良纳米载体,具有良好的应用价值。
OBJECTIVE To prepare amphotericin B PLA-pullulan nanomicelles and evaluate in vitro. Methods amphotericin B nano-micelles were prepared by the membrane hydration method using a novel amphiphilic polymer PLA-pullulan as carrier material. The morphology, morphology and morphology of micelles were characterized by transmission electron microscopy, laser particle size analyzer and zeta potential analyzer, respectively. Particle size and surface potential were characterized, determination of the entrapment efficiency, drug loading and in vitro release kinetics, investigate the drug dispersion state and hemolytic toxicity. Results The prepared amphotericin B polylactic acid pullulan nanomicellar solution was transparent and spherical under electron microscopy. The average particle size was 152.1 nm, the entrapment efficiency was 71.1%, and the drug loading was 10.9%. 24 h cumulative release of 10.01%, hemolytic toxicity than amphotericin sodium deoxycholate micelles. Conclusions Pullulan polylactic acid micelles is an excellent nanocarrier for amphotericin B, which has good application value.