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目的:建立猪肝脏中盐酸氯丙嗪残留的高效液相色谱法(HPLC)分析方法。方法:样品经酸性乙腈提取,过活化后的碱性氧化铝柱,浓缩混匀后,用高效液相色谱法测定。结果:盐酸氯丙嗪标准曲线在0.2μg/ml～1.5μg/ml浓度范围线性良好,线性相关系数(r)为0.9998;回收率在93.6%～113.3%之间,相对标准偏差(RSD)均小于7%(n=6);检测限为0.1 mg/kg,定量限为0.3 mg/kg。结论:本方法快速、准确,具有较低的检出限,较好的回收率和精密度,符合分析测定的要求,可用于动物性样品中盐酸氯丙嗪的检测。
Objective: To establish a HPLC method for the determination of chlorpromazine hydrochloride in pig liver. Methods: The samples were extracted with acidic acetonitrile and activated alumina column. After concentration and concentration, the samples were determined by high performance liquid chromatography. Results: The calibration curve of chlorpromazine hydrochloride had a good linearity in the range of 0.2μg / ml ~ 1.5μg / ml with a linear correlation coefficient (r) of 0.9998. The recoveries ranged from 93.6% to 113.3%. The relative standard deviations (RSDs) Less than 7% (n = 6). The detection limit was 0.1 mg / kg and the limit of quantification was 0.3 mg / kg. Conclusion: The method is rapid, accurate, has a lower detection limit, better recovery and precision, in line with the requirements of analysis and determination, can be used for the detection of chlorpromazine hydrochloride in animal samples.