论文部分内容阅读
近年来有关检测人血清Ⅲ型前胶原(human procollagen typeⅢ,hPCⅢ)含量诊断肝纤维化及肝硬化的文章颇多,而其它疾病hPCⅢ的含量变化则少有报道。我们应用hPCⅢRIA kit测定35例脑梗塞患者血清hPCⅢ含量,并对结果进行初步评价。 材料和方法 一、正常对照组:29例(男17例,女12例),均为全面体检合格的健康人,心、肝、肾功能正常,年龄45～63岁,平均51.2岁。 二、脑梗塞组:35例(男24例,女11例),全部病例均经临床和/或CT确诊,肝炎病毒血清学标志均阴性,肝功能正常。年龄53～82岁,平均67.4岁。 三、方法:受检者早晨空腹采血2ml,离心取血清-20℃保存,一周内同时检测hPCⅢ和血β_2-m。hPCⅢ试剂盒由重庆市肿瘤研究所提供。β_2-试剂盒由中国原子能科学研究院同位素所提供。测定仪器为中国科学院上海原子核研究所日环仪器厂SN-682B型微电脑)γ-计数仪。操作按说明书。同
In recent years, there are many articles about the detection of human procollagen type Ⅲ (hPCⅢ) in the diagnosis of liver fibrosis and cirrhosis, while the changes of hPCⅢ in other diseases are seldom reported. We use hPC Ⅲ RIA kit determination of 35 patients with cerebral infarction serum hPC Ⅲ levels, and the results of a preliminary evaluation. Materials and Methods First, the normal control group: 29 cases (17 males and 12 females), all of them were all healthy people with normal physical examination. Their heart, liver and kidney function were normal. They were 45 to 63 years old with an average of 51.2 years old. Second, cerebral infarction group: 35 cases (24 males and 11 females), all cases were confirmed by clinical and / or CT, hepatitis virus serological markers were negative, normal liver function. Age 53 ~ 82 years old, average 67.4 years old. Third, the method: the subjects fasting morning blood 2ml, centrifuged serum -20 ℃ preservation, simultaneous detection of hPC Ⅲ and blood β_2-m within a week. hPC Ⅲ kit provided by the Chongqing Institute of Oncology. β_2-kit by the China Institute of Atomic Energy isotopes provided. Measurement equipment for the Chinese Academy of Sciences, Shanghai Institute of Atomic Radio Day Instrument Factory SN-682B-type microcomputer) γ-counter. Operation according to the instructions. with