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Objective To study the expression of B7-1 protein in biopsies in allograft renal transplantation,and explore the expression patern and the functional role of B7-1 molecule. Methods Renal allograft sample tissue by Tru-cut needle aspiration were taken from 64 paients(42 male,22 female) with renal transplantation, aging from 17 to 58 years old; 64 cases were categorized into six groups: ①acute rejection (AR n =22);②accelerated rejection (AAR n =8);③chronic rejection (CR n =10 );④hyperacute rejection (HR n =4);⑤allograft with stable function (ASF n =10); ⑥health donor kidney (HDK n =10);Immunohistochemical assays(ABC) were used, and comparison B7-1 and HLA-DR expression between tubularand interstitial,the number of interstitial infiltrating lymphocytes. Results ①The sequence of the expression of B7-1 molecule in terms of intensity from the strongest to the weakest in different groups is AR group, AAR group, CR group, HR group,ASF group and HDK group; ②During acute rejection response, a large number of CD4 and CD8 T lymphocytes infiltrate kidney interstitium, accompanied by strong expression of HLA-DR in tubular cell(donor MHC-Ⅱantigen),which is the first signal necessary for T lymphocyte activation. Conclusion The results demonstrate that B7-1 expression of tubular cells as APC actively take part in immunological reactions and play an important role in expanding the immunological reactions.
Objective To study the expression of B7-1 protein in biopsies in allograft renal transplantation, and explore the expression patern and the functional role of B7-1 molecule. Methods Renal allograft sample tissue by Tru-cut needle aspiration were taken from 64 paients (42 (AR n = 22); ②accelerated rejection (AAR n = 8); ③chronic rejection (CR n = 10 ); Histotypes of HLA-DR expression (HbA1); ④ hyperacute rejection (HR n = 4); ⑤ allograft with stable function (ASF n = 10) results ① The sequence of the expression of B7-1 molecule in terms of intensity from the strongest to the weakest in different groups is AR group, AAR group, CR group, HR group, ASF group and HDK group; ②During acute rejection response, a large number of CD4 and CD8 T lymphocytes infiltrate kidney interstitium, accompanied by strong expression of HLA-DR in tubular cell (donor MHC-II antigen), which is the first signal necessary for T lymphocyte activation. Conclusion The results demonstrates that B7-1 expression of tubular cells as APC actively take part in immunological reactions and play an important role in expanding the immunological reactions.