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目的探讨凋亡抑制基因bcl-2蛋白及血管内皮生长因子(VEGF)表达在乳腺癌中的意义,及其与影响乳腺癌预后因素的相关性,探讨二者在细胞癌变过程中的作用及意义。方法应用免疫组织化学的方法,对64例随访5年以上的乳腺癌、19例良性乳腺组织、12例乳腺不典型增生的bcl-2蛋白、VEGF、雌激素受体(ER)、孕激素受体(PR)及C-erbB-2进行检测。结果64例乳腺癌组织中,bcl-2蛋白表达的总阳性率为65.6%(42/64),bcl-2高表达的病例,组织学分级高,易出现腋淋巴结转移;VEGF在乳腺癌组织中总的阳性表达率为62.5%(40/64),与肿瘤体积大、TNM分期晚、高组织学分级、腋淋巴结转移、ER阴性、C-erbB-2阳性等预示预后差等因素有显著相关性;乳腺癌中bcl-2与VEGF表达呈正相关(r=0.4172,P=0.0001)。在正常乳腺组织、乳腺不典型增生组织中bcl-2蛋白、VEGF表达的阳性率均低于乳腺癌组织,差异有显著性(P<0.01)。结论Bcl-2表达失调可导致乳腺癌细胞凋亡调控紊乱,bcl-2基因影响肿瘤的生物学行为;VEGF参与了乳腺癌的侵袭与转移调节,VEGF高表达常提示预后差;bcl-2基因可能通过调节细胞的凋亡而参与乳腺癌的发生发展;VEGF与bcl-2的表达呈正相关,VEGF是否能上调bcl-2蛋白有待进一步研究证实。
Objective To investigate the significance of apoptosis-inhibiting gene bcl-2 protein and vascular endothelial growth factor (VEGF) expression in breast cancer and its relationship with the prognosis of breast cancer, and to explore their roles and significance in the carcinogenesis of breast cancer . Methods Immunohistochemistry was used to detect the expression of bcl-2 protein, VEGF, estrogen receptor (ER) and progesterone receptor in 64 cases of breast cancer with more than 5 years of follow-up, 19 cases of benign breast tissue, 12 cases of breast dysplasia, Body (PR) and C-erbB-2 were detected. Results The positive rate of bcl-2 protein expression was 65.6% (42/64) in 64 cases of breast cancer tissues. The high expression of bcl-2 was associated with high histological grade and axillary lymph node metastasis. The expression of VEGF in breast cancer tissues The overall positive rate was 62.5% (40/64), which was significantly higher than the tumor size, TNM stage, histological grade, axillary lymph node metastasis, ER-negative and C-erbB-2 positive Correlation; There was a positive correlation between the expression of bcl-2 and VEGF in breast cancer (r = 0.4172, P = 0.0001). The positive rate of bcl-2 protein and VEGF in normal breast tissue and atypical hyperplasia tissues were lower than that in breast cancer tissues, the difference was significant (P <0.01). CONCLUSION: Bcl-2 gene may play an important role in the regulation of breast cancer cell apoptosis. Bcl-2 gene may affect the biological behavior of breast cancer. VEGF may be involved in the invasion and metastasis of breast cancer. It may be involved in the development of breast cancer by regulating cell apoptosis. The expression of VEGF and bcl-2 is positively correlated. Whether VEGF can up-regulate bcl-2 protein remains to be confirmed by further study.