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AIM:To investigate the role of tri-iodothyronine supplementin protecting gut barrier in septic rats.METHODS:Twenty-two rats were randomized into threegroups:sham group(n=6),sepsis group(n=8),and sepsisplus tri-iodothyronine(T_3)group(n=8).Septic rat modelwas established through cecal ligation and puncture(CLP).After 5 h,sham and sepsis groups received saline,and theremaining group received T_3 intraperitoneally.Twenty-onehrs After CLP,intestinal permeability and serum free T_3 andT4 were measured with fluorescence Spectrophotometer andby radioimmunoassay,respectively.Intestinal ultrastructureand histologic morphology were observed under transmissionelectron microscopy(TEM)and light microscopy,respectively.RESULTS:After 21 h,septic symptoms and signs in sepsisplus T_3 group were milder than those in sepsis group.SerumFT_3 or FT_4 concentration in sepsis group was lower than thatin sham group(1.59±0.20,3.41±2.14 pmol/L vs3.44±1.40,9.53±3.39 pmol/L,P<0.05),and FT_3 concentration in sepsisplus T_3 group(3.40±1.65 pmol/L,P<0.05)was corrected.Portal concentration of fluorescein isothiocyanate-dextran(FITC-D)in sepsis group(2.51±0.56 mg/L)was higher thanthat in sham group(1.22±0.21 mg/L)(P<0.01),and in sepsisplus T_3 group(1.68±0.38 mg/L)it was decreased significantly(P<0.01).TEM and light microscopy showed that T_3supplement preserved well ultrastructure and morphologyof intetinal mucosa in septic rats.CONCLUSION:Tri-iodothyronine supplement protects gutbarrier in septic rats.
AIM: To investigate the role of tri-iodothyronine supplement in protecting gut barrier in septic rats. METHODS: Twenty-two rats were randomized into three groups: sham group (n = 6), sepsis group (n = 8), and sepsisplus tri-iodothyronine Septic rat model was established through cecal ligation and puncture (CLP). After 5 h, sham and sepsis groups received saline, and theremaining group received T_3 intraperitoneally.Twenty-onehrs After CLP, intestinal permeability and serum free T_3 and T4 were measured with fluorescence Spectrophotometer andby radioimmunoassay, respectively.Intestinal ultrastructure and histologic morphology were observed under transmission electron microscopy (TEM) and light microscopy, respectively.RESULTS: After 21 h, septic symptoms and signs in sepsisplus T_3 group were milder than those in sepsis group. Serum FT_3 or FT_4 concentration in sepsis group was lower than that of sham group (1.59 ± 0.20, 3.41 ± 2.14 pmol / L vs 3.44 ± 1.40, 9.53 ± 3.39 pmol / L, P < se Psisplus T_3 group (3.40 ± 1.65 pmol / L, P <0.05) was corrected.Portal concentration of fluorescein isothiocyanate-dextran (FITC-D) in sepsis group (2.51 ± 0.56 mg / L) was higher thanthat in sham group 0.21 mg / L) (P <0.01), and in sepsisplus T_3 group (1.68 ± 0.38 mg / L) it was decreased significantly (P <0.01) .TEM and light microscopy showed that T_3supplement preserved well ultrastructure and morphology of intetinal mucosa in septic rats.CONCLUSION: Tri-iodothyronine supplement protects gutbarrier in septic rats.