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目的研究人参多糖对大鼠卵巢功能的作用。方法采用体外细胞培养观察人参多糖对HCG诱导的黄体与颗粒细胞分泌孕酮与cAMP的影响,并检测了卵母细胞的存活率。分离培养黄体、颗粒与卵母细胞,设对照组与不同浓度人参多糖实验组,采用放免分析方法测定孕酮与cAMP生成含量,细胞存活率检测采用MTT法。结果与对照组比较,孕酮含量:黄体细胞为(6573.46±185.14)与(4509.55±126.82)pmol,颗粒细胞为(197.16±42.87)与(320.42±12.46)pmol。cAMP:黄体细胞为(31.20±17.13)与(65.26±15.93)fmol,颗粒细胞为(121.15±19.96)与(296.42±27.28)fmol。结论人参多糖抑制HCG诱导黄体细胞孕酮分泌,促进HCG诱导颗粒细胞孕酮分泌。而协同黄体细胞与颗粒细胞cAMP生成,人参多糖使卵母细胞生长抑制率降低,呈区间剂量依赖关系。
Objective To study the effect of ginseng polysaccharide on ovarian function in rats. Methods The effect of ginseng polysaccharide on HCG-induced secretion of progesterone and cAMP from corpus luteum and granulosa cells was observed by in vitro cell culture, and the survival rate of oocytes was examined. The corpus luteum, granules and oocytes were isolated and cultured. The control group and the different concentrations of ginseng polysaccharide experimental group were used. The progesterone and cAMP production levels were determined by radioimmunoassay. The cell survival rate was measured by MTT assay. Results Compared with the control group, progesterone levels were (6573.46±185.14) and (4509.55±126.82) pmol for luteal cells, (197.16±42.87) and (320.42±12.46) pmol for granulosa cells. The cAMP: luteal cells were (31.20±17.13) and (65.26±15.93) fmol, and the granulosa cells were (121.15±19.96) and (296.42±27.28) fmol. Conclusion Ginseng polysaccharide can inhibit HCG-induced progesterone secretion in luteal cells and promote HCG-induced granulocyte progesterone secretion. In concert with the formation of cAMP from luteal cells and granulosa cells, the growth inhibition rate of ginseng polysaccharides was reduced, showing a dose-dependent relationship.