胆红素生产中的脱脂方法与酸化度研究

• 作 者: 黄仁术
• 机 构: 皖西学院化学与生命科学系,
• 出 处: 药物生物技术
• 发表日期: 2010年02期
• 关键词: 胆红素 快速法 脱脂方法 酸化度

为提高胆红素生产效益,采取正交试验优化筛选脱脂方法(双层纱布法、石油醚洗涤法、混合法)和酸化度(pH5.0、pH5.5、pH6.0),结果表明:脱脂方法和酸化度对胆红素提取差异高度显著(P<0.01),影响度脱脂方法>酸化度;在双层纱布过滤、酸化至pH5.0时萃取的常规条件下,胆红素的粗制品收率0.0923 g/L、纯度36.18%,折合纯品收率0.0333 g/L;在混合法脱脂、酸化至pH5.5时萃取的条件下,胆红素的粗制品收率0.6122 g/L、纯度52.05%,折合纯品收率最高,达0.3186 g/L。

In order to improve the production efficiency of bilirubin, orthogonal test was used to optimize the method of degreasing (double gauze method, petroleum ether washing method, mixing method) and acidification degree (pH5.0, pH5.5, pH6.0). The results showed that: The method of degreasing and the degree of acidification were highly significant (P <0.01), the degree of degreasing method> degree of acidification. Under the condition of double gauze filtration and acidification to pH 5.0, the crude bilirubin The product yield was 0.0923 g / L, the purity was 36.18%, which was equivalent to the pure product yield of 0.0333 g / L. Under the conditions of degreasing and acidification to pH5.5 by extraction, the yield of crude product of bilirubin was 0.6122 g / L , Purity of 52.05%, equivalent to the highest yield of pure products, up to 0.3186 g / L.