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[目的 ]论证我国雷氏按蚊与嗜人按蚊的分类地位。 [方法 ]采用分子鉴别法 (鉴别PCR和rDNA ITS2序列 )检测辽宁及山东两省现场按蚊标本 ,并依据ITS2区序列建立分子系统树。 [结果 ]分子鉴别显示 ,辽宁和山东两省均存在雷氏按蚊和嗜人按蚊。我国雷氏按蚊 (辽宁和山东省 ,n =6 )和嗜人按蚊 (辽宁、云南、河南和四川省 ,n =10 )ITS2序列长度和GC含量分别为 45 1bp、 46 2 % ,44 8bp、 46 0 % ;各地雷氏按蚊和嗜人按蚊序列均无差异 ,但各地嗜人按蚊与对照组江苏的嗜人按蚊相比 ,种内序列差异为 0 88% ;雷氏按蚊与嗜人按蚊种间序列差异为 2 5 7%。分子系统树显示雷氏按蚊与中华按蚊、嗜人按蚊与凉山按蚊亲缘关系较近。 [结论 ]根据分子鉴别 ,确认我国雷氏按蚊与嗜人按蚊为同域分布的 2个独立种
[Objective] To demonstrate the taxonomic status of An. [Method] The Anopheles mosquitoes samples from Liaoning and Shandong provinces were detected by molecular identification (PCR and rDNA ITS2 sequences) and the molecular phylogenetic tree was constructed based on ITS2 sequences. [Result] The molecular identification showed that An. And An. An. Oppositus existed in Liaoning and Shandong provinces. ITS2 sequence length and GC content in Anopheles sinensis (Liaoning and Shandong provinces, n = 6) and Anopheles anthropophagus (n = 10 in Liaoning, Yunnan, Henan and Sichuan provinces) were 451 bp and 462% respectively 8bp and 46 0%, respectively. There was no difference in the sequences of An. Anopheles and Anopheles anthropophagus, but the An. Anopheles populations in Jiangsu were 0.88% The difference between Anopheles anthropophagus and Anopheles anthropophagus was 25.7%. Molecular phylogenetic tree shows that Anopheles rexiana and Anopheles sinensis, Anopheles anthropophagus and Anopheles mosquitoes are more closely related. [Conclusion] According to the molecular identification, it was confirmed that An. Mosquito and Anopheles anthropophagus were two independent species