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目的:观察三七总皂苷对家兔离体小肠平滑肌收缩活动的影响,并探讨其作用机制。方法:取健康家兔,雌雄不拘,将小肠离体后恒温灌流,观察三七总皂苷对家兔小肠自发收缩活动的影响;在灌流液中分别加入BayK8644、左旋硝基精氨酸甲酯(L-NAME)后再加入三七总皂苷,研究其作用机制;在无钙台式液中加入rynodine后再加入三七总皂苷,研究其作用机制。结果:三七总皂苷剂量依赖性的抑制家兔离体小肠平滑肌收缩的幅度。BayK8644和L-NAME均可完全阻断三七总皂苷对家兔小肠平滑肌收缩活动的抑制作用。在无钙台式液中,三七总皂苷显著抑制rynodine引起的细胞内钙收缩活动。结论:三七总皂苷显著抑制家兔小肠平滑肌的收缩活动,其抑制收缩活动机制可能是:增加小肠平滑肌NO浓度,从而抑制细胞外钙内流和内钙释放。
Objective: To observe the effects of Panax notoginseng saponins on contractile activity of isolated small intestine smooth muscle in rabbits and to explore its mechanism. Methods: Healthy rabbits were randomly divided into male and female rabbits. The small intestine was isolated and perfused with constant temperature to observe the effect of Panax notoginseng saponins on spontaneous contractions of rabbit intestine. BayK8644, L-NAME L-NAME), and then added Panax notoginseng saponins to study the mechanism of action; add rynodine in calcium-free tabletop before adding Panax notoginseng saponins to study its mechanism of action. Results: Panax notoginsenoside dose-dependently inhibited the contraction of isolated rabbit intestinal smooth muscle. Both BayK8644 and L-NAME completely blocked the inhibitory effect of Panax Notoginseng Saponins on contractile activity of rabbit intestinal smooth muscle. Panax notoginseng saponins significantly inhibited rynodine-induced intracellular calcium contractile activity in calcium-free tabletops. Conclusion: Panax notoginsenoside can significantly inhibit the contractile activity of rabbit intestinal smooth muscle. Its mechanism of inhibiting contractile activity may be: increase the concentration of NO in intestinal smooth muscle, and thus inhibit the extracellular calcium influx and intracellular calcium release.