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建立稳定表达Smad蛋白的MDPC -2 3细胞克隆。方法 :将Smad表达载体转染进MDPC -2 3细胞内 ,通过G418筛选出阳性克隆 ,用Flag抗体进行Westernblot鉴定。 结果 :表达Flag融合蛋白细胞克隆为稳定整合Smad基因的阳性克隆 ,不表达Flag融合蛋白细胞克隆为表达空载体的细胞克隆。结论 :获得稳定表达Smad的细胞克隆 ,为以后研究Smad在成牙本质细胞分化中的作用奠定了基础
Establish MDPC -23 cell clone stably expressing Smad protein. Methods: The Smad expression vector was transfected into MDPC-23 cells. The positive clones were screened by G418 and identified by Flag blot. RESULTS: The Flag fusion protein cell clone was a positive clone stably integrating the Smad gene, and the Flag fusion protein cell clone was not expressed as a cell clone expressing the empty vector. Conclusion: Obtaining stable cell clones expressing Smad lays the foundation for the future study of the role of Smad in odontoblast differentiation