论文部分内容阅读
本文用噻唑盐比色方法探讨在 60 Co辐射损伤条件下扇贝多肽对胸腺细胞的保护作用 ,和对 60 Co辐射损伤的胸腺细胞修复能力的影响。结果表明 :(1) 60 Co辐射后胸腺细胞的活性明显下降。 (2 )先加扇贝多肽后辐射组的胸腺细胞的活性较未加扇贝多肽的辐射组强 (P<0 .0 5 ) ,0 .2 5 %～ 4 %浓度范围内 ,胸腺细胞的活性与扇贝多肽的浓度呈正比。当辐射强度加大到 9～ 10 GY时 ,0 .5 %以下的扇贝多肽保护能力减弱或丧失。(3)先 60 Co辐射后 2 h,再加入扇贝多肽孵育 ,胸腺细胞的活性随扇贝多肽浓度的加大而增强。辐射后 7h或 19h再加入扇贝多肽 ,仅在辐射 7h后加入 2 %的扇贝多肽孵育的胸腺细胞的活性较对照组活性强 ;辐射后 19h再加入扇贝多肽组的胸腺细胞的活性均较对照组显著降低 (P<0 .0 5 )。结果提示 :提示扇贝多肽具有抵抗 60 Co辐射对胸腺细胞的损伤作用 ,并且呈剂量依赖性 ;而且在一定的时间范围内对受到辐射损伤后的胸腺细胞具有促进其修复的作用
In this paper, the thiazolium colorimetric method to explore the 60Co radiation damage scallop peptides on thymocyte protective effect, and 60Co radiation damage thymocytes repair ability. The results showed that: (1) The activity of thymocytes after 60 Co radiation decreased obviously. (2) The activity of thymocytes in radiation group after adding scallop peptides was stronger than that in radiation group without scallop peptides (P <0.05), and the activity of thymocytes in 0.25% ~ 4% Scallop peptide concentration was proportional. When the radiation intensity increased to 9 ~ 10 GY, 0.5% scallop polypeptide protection ability weakened or lost. (3) After 60Co irradiation for 2h, scallop peptides were incubated again, and the activity of thymocytes increased with the increase of scallop peptides concentration. Scallop peptides were added at 7h or 19h after radiation, and thymocytes incubated with 2% scallop peptides only after 7h irradiation were more active than those in control group. The activity of thymocytes in scallop peptide group after 19h irradiation was higher than that in control group Significantly lower (P <0.05). The results suggest that: scallop peptides have the effect of inhibiting the damage of thymocytes by 60 Co radiation in a dose-dependent manner, and have the effect of promoting the repair of thymocytes after radiation injury within a certain time range