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本文作者依据酶标(EIA)测定乙型肝炎表面抗原(HBsAg)的方法,制成抗体电极,来测定生物液中的HBsAg.其方法是:首先分离提纯人血清中HBsAg,用以免疫家兔,制备特异性的抗体。分离纯化抗体,先用电泳,再用二乙基氨基乙基纤维素(DEAE-Cellulose)柱层析。采用NaKace等人的方法,将所得家兔抗HBsAg的γ球蛋白与辣根过化氧物酶连接。所得的抗体-酶的复合物,用葡聚糖G200分离提纯。用403nm的波长测定。按2-3个分子的辣根过氧化物酶与一个分子的IgG结合,将未标记的抗体,固定到人工膜上后,将其浸泡在每升含1克的庆大霉素,pH6.80 20mmol/L的磷酸
According to the method of enzyme-linked immunosorbent assay (EIA) for the determination of hepatitis B surface antigen (HBsAg), an antibody electrode is prepared for the determination of HBsAg in biological fluids by first isolating and purifying human serum HBsAg to immunize rabbits , To prepare a specific antibody. The antibody was isolated and purified by electrophoresis and then by DEAE-Cellulose column chromatography. Using the method of NaKace et al., The resulting rabbit anti-HBsAg gamma globulin was ligated with horseradish peroxidase. The resulting antibody-enzyme complex was isolated and purified using dextran G200. Measured at 403 nm. After binding 2-3 molecules of horseradish peroxidase to one molecule of IgG, the unlabeled antibody is immobilized on an artificial membrane and then soaked in 1 g of gentamycin, pH 6, per liter. 80 20 mmol / L phosphoric acid