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目的研究三七总皂苷(panax notoginseng saponin,PNS)抗心肌细胞肥大是否与成纤维细胞生长因子诱导早期反应蛋白14(fibroblast growth factor-inducible 14,Fnl4)的表达变化相关。方法分离原代新生大鼠心肌细胞,去甲肾上腺素(Norepinephrine,NE)处理原代细胞建立心肌细胞肥大模型。鬼笔环肽染色观察细胞形态,实时定量聚合酶链反应(quantitative real-time polymerase chain reaction,q RT-PCR)检测肥大相关基因和Fn14 m RNA表达水平,Western blot检测Fn14蛋白表达水平。结果与对照组比较,NE处理组细胞表面积增大、细胞肥大相关基因m RNA表达水平明显升高,Fn14 m RNA和蛋白表达升高,差异均有统计学意义(P<0.05)。与单独NE组比较,NE和PNS同时处理组的细胞表面积减小、肥大相关基因m RNA表达水平显著下降,差异有统计学意义(P<0.05)。PNS对Fn14 m RNA表达水平影响不大,但是却明显的促进Fn14蛋白表达(P<0.05)。结论 PNS能够抗NE诱导的心肌细胞肥大,同时能够影响Fn14的表达。PNS抗心肌肥大可能通过Fn14起作用。
Objective To investigate whether cardiomyocyte hypertrophy associated with panax notoginseng saponin (PNS) is related to the expression of fibronectin-induced early fibrotic growth factor-inducible 14 (Fnl4). Methods Primary neonatal rat cardiomyocytes were isolated and primary cells were treated with norepinephrine (NE) to establish cardiomyocyte hypertrophy model. Phalloidin staining was used to observe the cell morphology. The mRNA and protein levels of Fn14 mRNA were detected by quantitative real-time polymerase chain reaction (q RT-PCR). The expression of Fn14 protein was detected by Western blot. Results Compared with the control group, the cell surface area of NE-treated group increased, the mRNA expression of mH-related gene was significantly increased, and the expression of Fn14 mRNA and protein were increased. The difference was statistically significant (P <0.05). Compared with the NE alone group, the cell surface area of the NE and PNS treated groups decreased at the same time, and the mRNA expression level of hypertrophy related gene significantly decreased (P <0.05). PNS had little effect on Fn14 mRNA expression, but significantly enhanced Fn14 protein expression (P <0.05). Conclusion PNS can prevent cardiomyocyte hypertrophy induced by NE and can affect the expression of Fn14. PNS anti-cardiac hypertrophy may play a role through Fn14.