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用微核试验法检测氮氧喹呱的致突变作用,预测致癌危险性,确定剂量—反应关系。取本省繁殖小鼠,体重分布在20～24克之间,随机分为六组(1/6LD_(50)组、1/12LD_(50)组、1/24LD_(50)组、1/48LD_(50)组,阳性和阴性对照组),于染毒后24小时取骨髓液制片,观察多染红细胞中微核数。结果发现,各组微核均呈园形或杏仁状结构,氮氧喹呱组微核出现率低于阳性对照组(P<0.01),与阴性对照组接近(P>0.05),氮氧喹呱各组间微核率似有随剂量增加而提高趋势,但无显著差别,此与文献报导Ames试验结果一致,据此认为氮氧喹呱无致突变作用,可排除其致癌危险性。
Using micronucleus test method to test the mutagenic effect of oxirapurine to predict the carcinogenic risk and determine the dose-response relationship. The mice were randomly divided into six groups (1 / 6LD 50, 1 / 12LD 50, 1 / 24LD 50, 1 / 48LD 50 ) Group, positive and negative control group). The bone marrow was prepared 24 hours after exposure to observe the micronuclei in polychromatic erythrocytes. The results showed that the micronuclei in each group showed a round or almond-shaped structure, and the occurrence rate of micronuclei in the nitrooxyquinux group was lower than that in the positive control group (P <0.01), close to the negative control group (P> 0.05) The rates of micronuclei in each group seemed to increase with the increase of dosage, but there was no significant difference. It was consistent with the Ames test reported in the literature. Therefore, no any mutagenic effect was found and no carcinogenic risk could be ruled out.