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目的及时发现河北省可能出现的脊灰疫苗衍生病例(VDPV)。方法对河北省2007-2008年急性弛缓性麻痹(AFP)病例的粪便标本进行病毒分离和血清定型。所有标本用L20B、RD细胞同时进行病毒分离,用PCR-RFLP法做型内鉴定。结果从报告的763例AFP病例粪便标本中分离到42株脊髓灰质炎病毒株(PV),其中Ⅰ型4株,Ⅱ型11株,Ⅲ型11株,混合型13株,PV+NEPV共3株;将混合株进行单型分离后,共得到58株,以Ⅲ型PV为主,均为疫苗变异株;发病年龄在1岁以内儿童占84.48%,男性发生变异39株,女性发生19株。结论河北省2007-2008年分离到的脊髓灰质炎疫苗病毒有部分变异,未发现疫苗衍生脊灰病毒。
Objective To discover the possible poliovirus derivative cases (VDPV) in Hebei Province in time. Methods Stool samples of acute flaccid paralysis (AFP) cases from 2007 to 2008 in Hebei province were isolated and serotyped. All specimens with L20B, RD cells simultaneously virus isolation, PCR-RFLP method of intra-type identification. Results A total of 42 poliovirus strains were isolated from 763 AFP cases, including 4 strains of type Ⅰ, 11 of type Ⅱ, 11 of type Ⅲ and 13 of mixed type. There were 3 PV + NEPV A total of 58 strains were obtained after the single type separation of the mixed strains. The type Ⅲ PVs were the main vaccine strains. The age of onset was within 1 year of age, accounting for 84.48%. There were 39 males and 19 females, . Conclusion The poliovirus isolated from 2007-2008 in Hebei Province showed partial variation and no vaccine-derived poliovirus was found.