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本研究通过对深圳市4名甲流重症患者的血清抗体及其所感染的新甲型H1N1流感病毒的抗原性和分子特点的分析,发现这些患者在感染后短期内产生的血清中和抗体滴度均不超过1:20,不能起到有效的保护作用;交叉血凝抑制实验的结果显示新H1N1病毒与季节性H1N1和H3N2流感病毒无任何交叉反应,抗原性差异很大,而患者所感染的病毒与标准株的抗原性则没有太大差异;分子特点的分析表明新H1N1病毒进入人群后依然属于经典的猪流感亚系,4名重症患者感染的病毒不具备高致病性流感病毒的遗传特点,几个氨基酸位点的变异没有影响病毒的毒力和致病性,只有一株毒株的NA蛋白发生了His275Tyr的突变,产生了对达菲等神经氨酸酶抑制剂的耐药性。
In this study, we analyzed the antigenicity and molecular characteristics of four Serum A patients with severe influenza A in Shenzhen and their Influenza A (H1N1) viruses and found that serum neutralizing antibody drops produced shortly after infection in these patients Degree of no more than 1:20, can not play an effective protective effect; results of cross-haemagglutination inhibition test showed that the new H1N1 virus and the seasonal H1N1 and H3N2 influenza virus without any cross-reaction, antigenic differences vary greatly, and the patient is infected Of the virus and the standard strain of antigenicity is not much difference; molecular characteristics of the analysis shows that the new H1N1 virus into the crowd still belong to the classic swine flu subfamily, four patients with severe infection of the virus does not have the highly pathogenic influenza virus Genetic characteristics, a few amino acid changes in the site did not affect the virulence and pathogenicity of the virus, only a strain of NA protein His275Tyr mutation occurred, resulting in Tamiflu and other neuraminidase inhibitors Sex.