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斜面菌种在试管之间的多次转接扩大,是由菌丝体到菌丝体连续进行的无性繁殖的过程,不产生子实体和有性孢子,不存在有性繁殖世代,所以不能把转管几次说成是转代几次,而把转管一次解释为继代一次是比较确切的。斜面菌种的多次转管继代,历来有不同的观点。一种认为,随着转次增多,容易导致种性的衰退,因而主张母种的首次分离量要大,以避免转次的增多,而且一般认为转管次数以3～4次为宜,所以通常按转管的先后次序,又有一级母种、二级母种……
Beveled species in the test tube between the transfer of multiple expansion is from mycelium to mycelium continuous asexual reproduction process, does not produce fruiting bodies and sexual spores, there is no sexual reproduction generation, it can not be Pipe a few times that is a few times, and the pipe was interpreted as the next generation is more accurate. Beveled strains of pipe transfer generations, has always had a different point of view. One thinks, with the increase of transfer, easily lead to the recession of species, thus advocating the first separation of maternal species to be large, in order to avoid the increase of transfer, and generally considered the number of transfer to 3 to 4 times is appropriate, so Usually according to the order of pipe transfer, but also a mother, mother of two ...