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目的观察蛤蚧肽对S180和Hepa1-6荷瘤小鼠免疫调节及其对小鼠移植肿瘤的生长抑制作用。方法以S180和Hepa1-6荷瘤小鼠为模型,研究蛤蚧肽及其联合环磷酰胺(CTX)对肿瘤的治疗效果,采用MTS法测定小鼠腹腔巨噬细胞杀瘤活性、脾淋巴细胞刺激指数、自然杀伤细胞(NK)活性,并称瘤重,计算抑瘤率。结果单纯蛤蚧肽灌胃400 mg.kg-1.d-1,连续12 d,可显著提升S180荷瘤小鼠的腹腔巨噬细胞杀瘤活性及Hepa1-6荷瘤小鼠的腹腔巨噬细胞吞噬功能(P<0.05),蛤蚧肽治疗后S180及Hepa1-6荷瘤小鼠脾淋巴细胞增殖能力及NK细胞活性均显著提高(P<0.05)。与CTX联合应用时,蛤蚧肽能使受CTX抑制的上述免疫指标得到改善,并能显著提高抑瘤率。结论蛤蚧肽对肿瘤及化疗药物造成的免疫功能抑制有调节作用,并可通过此途径达到协同化疗药物抗肿瘤效果。
Objective To observe the immunomodulatory effects of Gekko gecko peptides on S180 and Hepa1-6 tumor-bearing mice and their inhibitory effects on tumor growth in mice. Methods S180 and Hepa1-6 tumor-bearing mice were used as models to study the therapeutic effect of Gekko gecko peptide and its combination with cyclophosphamide (CTX) on tumor. MTS method was used to determine the tumoricidal activity of murine peritoneal macrophages, splenic lymphocyte stimulation Index, natural killer (NK) activity, and said tumor weight, calculate the inhibition rate. RESULTS: Gemcitabine peptide was administered intragastrically at 400 mg.kg-1.d-1 for 12 consecutive days, which markedly enhanced the tumoricidal activity of peritoneal macrophages in S180-bearing mice and the peritoneal macrophages in Hepa1-6-bearing mice Phagocytosis (P <0.05). The proliferation and NK cell activity of splenic lymphocytes of S180 and Hepa1-6 tumor-bearing mice were significantly increased after treatment with Gekko gecko peptide (P <0.05). When used in combination with CTX, Gekko gecko peptides improved the above-mentioned immune parameters inhibited by CTX and significantly increased the tumor inhibition rate. Conclusion Gekko gecko peptides regulate the immune function of tumor and chemotherapeutic drugs, and can achieve the antitumor effect of chemotherapeutic drugs through this route.