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为了探讨线粒体ＤＮＡ（ｍｔＤＮＡ）在细胞癌变中的作用，实验采用一步法快速制备癌细胞系ｍｔＤＮＡ，用ＰｖｕⅡ、ＸｈｏⅠ、ＰｓｔⅠ、ＥｃｏＲⅠ、ＢｓｔＥⅡ、ＨｉｎｄⅢ、ＨｐａⅠ、ＢｃｌⅠ、ＥｃｏＲⅤ、ＳｃａⅠ和ＸｂａⅠ共１１种限制性内切酶对ＳＰＣ－Ａ－１细胞系的ｍｔＤＮＡ进行了限制性片段长度多态性（ＲＦＬＰ）分析，并根据单酶切及双酶切结果，构建了ＳＰＣ－Ａ－１细胞系ｍｔＤＮＡ限制性酶切图谱。结果发现，ＳＰＣ－Ａ－１细胞ｍｔＤＮＡ基因编码区的３２个酶切位点均无变异，仅在其ｍｔＤＮＡ非编码区第１６２７６位核苷酸处出现了ＥｃｏＲⅤ新的酶切位点。结果表明ＳＰＣ－Ａ－１细胞ｍｔＤＮＡ基因编码区结构相当稳定，而主要的核苷酸变异可能位于其ｍｔＤＮＡ非编码区
In order to investigate the role of mitochondrial DNA (mtDNA) in the carcinogenesis of cancer cells, the mtDNA of cancer cell lines was rapidly prepared by one-step method. The mtDNA of mtDNA was constructed by using a total of 11 species of PvuⅡ, XhoⅠ, PstⅠ, EcoRⅠ, BstEⅡ, HindⅢ, HpaⅠ, BclⅠ, EcoRⅤ, ScaⅠand XbaⅠ Restriction endonuclease restriction fragment length polymorphism (RFLP) analysis of mtDNA of SPC-A-1 cell line, and according to the results of single digestion and double digestion, the SPC-A-1 cell line mtDNA Restriction map. The results showed that the 32 restriction sites of the mtDNA gene coding region of SPC-A-1 cells showed no mutation, and only a new EcoRV restriction site was found at the nucleotide of 16276 in the non-coding region of mtDNA. The results showed that the coding region of mtDNA gene in SPC-A-1 cells was quite stable, while the main nucleotide variation might be located in the mtDNA non-coding region