论文部分内容阅读
目的:研究植入125I粒子后家兔气管组织结构的辐射损伤,探讨临床应用安全性。方法:采用2×2析因设计方法,将实验组和空白对照组动物在不同时间点(第14天,第30天)的损伤程度进行组合交叉分组,研究辐射和时间两种因素各自损伤水平,以及两者间有无交互作用。具体分组方法如下:健康雄性普通级新西兰家兔16只(体重2.5～3.0㎏)按照体重与窝别配为8对,再随机分为2个亚组(第14天组,第30天组),每组4对;每对动物各自分为实验组1只,对照组1只。实验组(8只)采取气管外膜缝扎3颗0.5mCi125I粒子,粒子互相间隔10mm排列的布源方法,对照组(8只)采用3颗空白粒子,按相同方法布源。第14天与第30天分别取相应亚组动物气管进行肉眼观察、HE染色病理与透射电镜检查,观察炎症损伤程度,进行统计分析。结果:第14天和第30天,实验组和对照组兔左侧气管均无穿孔、血栓形成等严重并发症;两组HE染色对比炎症损伤程度计分无显著性差异(P>0.05);实验组电镜观察到辐射敏感细胞器损伤,但第14天与第30天无明显程度差异,对照组电镜检查正常。结论:间隔10mm连续排列的3颗0.5mCi125I粒子对家兔气管的辐射损伤小,临床应用安全。
OBJECTIVE: To study the radiation damage of tracheal tissue of rabbits after implantation of 125I particles and to explore the safety of clinical application. Methods: 2 × 2 factorial design method was used to cross-group the injury degree of experimental group and blank control group at different time points (day 14 and day 30) to study the effects of irradiation and time on their respective injury levels , And whether the interaction between the two. The specific methods were as follows: Sixteen healthy male New Zealand rabbits (weighing 2.5-3.0 kg) were divided into 8 pairs according to body weight and litter size, and then randomly divided into 2 subgroups (day 14 and day 30) , Each group of 4 pairs; each animal was divided into experimental group 1, control group 1. In the experimental group (8), three 0.5 mCi125I particles were sewn through the tracheal outer membrane, and the cloths were arranged 10 mm apart. The control group (8 pcs) used three blank particles and distributed the same way. On the 14th day and the 30th day, the trachea of the corresponding subgroups were respectively examined by naked eye, HE staining and transmission electron microscopy. The degree of inflammatory injury was observed and statistically analyzed. Results: On the 14th and the 30th day, there were no serious complications such as perforation and thrombosis in the left trachea of the experimental group and the control group. There was no significant difference between the two groups in the severity of inflammatory injury (P> 0.05). In the experimental group, the radiation-sensitive organelles were observed by electron microscopy, but there was no significant difference between the 14th and the 30th days. The control group had normal electron microscopy. CONCLUSION: Three 0.5mCi125I particles arranged in succession at intervals of 10mm have less damage to the trachea of rabbits and their clinical application is safe.