【摘 要】
：
Little data is available on the evaluation of the occurrence rates of Epstein-Barr virus (EBV) in saliva and relationship with highly active antiretroviral therapy (HAART) use in HIV/AIDS patients in China.We conducted a retrospective cohort study of EBV
【机 构】
：
Center of Clinical Laboratory, The Fifth People’s Hospital of Wuxi, Affiliated to Jiangnan Universit
论文部分内容阅读
Little data is available on the evaluation of the occurrence rates of Epstein-Barr virus (EBV) in saliva and relationship with highly active antiretroviral therapy (HAART) use in HIV/AIDS patients in China.We conducted a retrospective cohort study of EBV serological tests for HIV/AIDS patients who were treated in the hospitals for infectious diseases in Wuxi and Shanghai,China from May 2016 to April 2017.The EBV-seropositive samples were identified by ELISA.EBV-specific primers and probes were used for the quantitative detection of viral DNA from saliva via quantitative real-time polymerase chain reaction.CD4 cell counts of the HIV/AIDS patients were detected by a flow cytometry.A total of 372 HIV/AIDS patients were ultimately selected and categorized for this retrospective cohort study.For EBV IgG and IgM,the HIV/AIDS HAART use (H) and non-HAART use (NH) groups had significantly higher seropositive rates than the HIV-negative control group.The HIV/AIDS (NH) group had the highest seropositive rate (IgG,94.27％;IgM,68.98％) and the highest incidence of EBV reactivation or infection.For salivary EBV DNA-positive rates and quantities,the HIV/AIDS (H) (73.69％) and the HIV/AIDS (NH) (100％) groups showed significantly higher values than the HIV-negative control group (35.79％,＞ twofold).Further,the salivary EBV DNA-negative population had significantly higher CD4 cell counts than the EBV DNA-positive population in the HIV/AIDS (H) group and the HIV/AIDS (NH) groups.Thus,HAART use is beneficial in decreasing the EBV salivary shedding in HIV/AIDS patients and indirectly decreases EBV transmission risk.
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