【摘 要】
：
The establishment of highly sensitive diagnostic methods is critical in the early diagnosis and control of Zika virus (ZIKV) and in preventing serious neurological complications of ZIKV infection.In this study,we established micro-droplet digital polymera
【机 构】
：
Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern
论文部分内容阅读
The establishment of highly sensitive diagnostic methods is critical in the early diagnosis and control of Zika virus (ZIKV) and in preventing serious neurological complications of ZIKV infection.In this study,we established micro-droplet digital polymerase chain reaction (ddPCR) and real-time quantitative PCR (RT-qPCR) protocols for the detection of ZIKV based on the amplification of the NS5 gene.For the ZIKV standard plasmid,the RT-qPCR results showed that the cycle threshold (Ct) value was linear from 101 to 108 copy/μtL,with a standard curve R2 of 0.999 and amplification efficiency of 92.203％;however,a concentration as low as 1 copy/μL could not be detected.In comparison with RT-qPCR,the ddPCR method resulted in a linear range of 101-104 copy/μL and was able to detect concentrations as low as 1 copy/μL.Thus,for detecting ZIKV from clinical samples,RT-qPCR is a better choice for high-concentration samples (above 101 copy/μL),while ddPCR has excellent accuracy and sensitivity for low-concentration samples.These results indicate that the ddPCR method should be of considerable use in the early diagnosis,laboratory study,and monitoring of ZIKV.
其他文献
Mycoviruses have been found to infect more than 12 species of Penicillium,but have not been isolated from Penicillium italicum (P.italicum).In this study,we isolated and characterized a new double-stranded RNA (dsRNA) virus,designated Penicillium italicum
Hand,foot and mouth disease (HFMD) is a major public health concern in China.The most predominant enteroviruses that cause HFMD have traditionally been attributed to enterovirus A71 (EVA71) and coxsackievirus A16 (CVA16).Since its first large outbreak in
The mechanism of how SARS-CoV-2 causes severe multi-organ failure is largely unknown.Acute kidney injury (AKI) is one of the frequent organ damage in severe COVID-19 patients.Previous studies have shown that human renal tubule cells could be the potential
Vectored vaccines based on highly attenuated modified vaccinia Ankara (MVA) are reported to be immunogenic,tolerant to pre-existing immunity,and able to accommodate and stably maintain very large transgenes.MVA is usually produced on primary chicken embry
Rabies is one of the most dangerous and widespread zoonosis and is characterized by severe neurological signs and a high case-mortality rate of nearly 100％.Vaccination is the most effective way to prevent rabies in humans and animals.In this study,the rel
Dear Editor,Marburg virus (MARV) belongs to the Filoviridae family,along with the related Ebola virus (EBOV).Although MARV is less renowned than EBOV,it causes an equally devastating disease,with clinical symptoms similar to EBOV infection and a remarkabl
Rice stripe mosaic virus (RSMV) is a rhabdovirus recently found in southern part of China and can cause severe reduction in rice production.To establish serological methods for RSMV epidemiological studies and to establish a control strategy for this viru
Porcine reproductive and respiratory syndrome virus (PRRSV) shows characteristic antibody-dependent enhancement (ADE) of infection and causes porcine systemic inflammation,which is similar to a type Ⅰ allergic reaction;however,the role of porcine FcεRI in
Despite the success of combined antiretroviral therapy in recent years,the prevalence of human immunodeficiency virus (HIV)-associated neurocognitive disorders in people living with HIV-1 is increasing,significantly reducing the health-related quality of